| 杨坤禹,刘莉,张涛,胡豫,Claudia Ruebe,Christian Ruebe,伍钢.MMPs/TIMPs系统在小鼠急性放射性肺损伤中的表达[J].中华放射医学与防护杂志,2006,26(4):360-363 |
| MMPs/TIMPs系统在小鼠急性放射性肺损伤中的表达 |
| Increased expressions of MMP-2 and MMP-9 in lung following 12 Gy local irradiation |
| 投稿时间：2006-01-18 |
| DOI： |
| 中文关键词: 基质金属蛋白酶(MMPs) 基质金属蛋白酶组织抑制物(TIMPs) 放射性肺损伤 |
| 英文关键词:Metalloproteinases (MMPs) Tissue inhibitors of metalloproteinases (TIMPs) Radiation-induced lung damage |
| 基金项目: |
| 作者 | 单位 | E-mail | | 杨坤禹 | 430023 武汉, 华中科技大学同济医学院附属协和医院肿瘤中心 | wanggangzr@yahoo.com.cn | | 刘莉 | 430023 武汉, 华中科技大学同济医学院附属协和医院肿瘤中心 | | | 张涛 | 430023 武汉, 华中科技大学同济医学院附属协和医院肿瘤中心 | | | 胡豫 | 430023 武汉, 华中科技大学同济医学院附属协和医院血液学研究所 | | | Claudia Ruebe | Department of Radiation Oncology Saarland University Hospital, Germary, Department of Radiation Oncology, Saarland University Hospital | | | Christian Ruebe | Department of Radiation Oncology Saarland University Hospital, Germary, Department of Radiation Oncology, Saarland University Hospital | | | 伍钢 | 430023 武汉, 华中科技大学同济医学院附属协和医院肿瘤中心 | |
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| 中文摘要: |
| 目的 通过检测基质金属蛋白酶(MMPs)和基质金属蛋白酶组织抑制物(TIMPs)在单次大剂量胸部照射小鼠肺组织中的表达,探讨全肺照射诱导的MMPs/TIMPs系统表达紊乱在放射性肺损伤发生与发展中的作用。方法 实验组小鼠在出生后8周时给予单次胸部照射12Gy,对照组小鼠给予假照射。在照射后4和8周时处死小鼠,收集肺组织标本,测定肺组织中MMP-2、MMP-9、MMP-3、MMP-13、TIMP-1、TIMP-2和TIMP-3蛋白的表达。结果 照射后4和8周时,实验组小鼠肺组织中MMP-2表达水平分别为对照组的1.7和1.9倍,MMP-9表达水平分别为对照组的2.7和2.6倍,而MMP-3、MMP-13、TIMP-1、TIMP-2、和TIMP-3的表达水平与对照组比较无显著改变。结论 辐射诱导小鼠肺组织中MMP-2和MMP-9的表达增加,可能加剧了急性放射性肺损伤。 |
| 英文摘要: |
| Objective To measure expressions of metalloproteinases and tissue inhibitors of metalloproteinases in the lung following thoracic irradiation of 12 Gy, and explore its possible role in the development of radiation-induced lung damage. Methods C57BL/6J mice at age of 8 weeks were thoracically irradiated with 12 Gy X-rays (10 MV, 2.4 Gy/min, single exposure), and the control mice were sham-irradiated. The mice were sacrificed at 4 or 8 weeks after thoracic iradiation by decapitation. Lung tissues samples were collected. Expressions of MMP-2, MMP-9, MMP-3, MMP-13, TIMP-1, TIMP-2, and TIMP-3 in lung samples were measured. Results There was no significant difference in expressions of MMP-3, MMP-13, TIMP-1 TIMP-2, and TIMP-3 in the lung between the two groups at 4 and 8 weeks after thoracic irradiation (or sham-irradiation). However, the expressions of MMP-2 were enhanced by 1.7 and 1.9 folds, and MMP-9 by 2.7 and 2.6 folds at 4 and 8 weeks after thoracic irradiation,respectively. Conclusion Enhanced expressions of MMP-2 and MMP-9 in the lung were involved in the development of acute lung injury after thoracic irradiation, leading to a disruption of the structure and fibrosis. |
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