| 王明明,朱巍,罗加林,等.AT细胞系辐射敏感性与细胞凋亡的相关研究[J].中华放射医学与防护杂志,2006,26(4):340-342.WANG Ming-ming,ZHU Wei,LUO Jia-lin,et al.Study on radiosensitivity of AT fibroblasts line using apoptosis[J].Chin J Radiol Med Prot,2006,26(4):340-342 |
| AT细胞系辐射敏感性与细胞凋亡的相关研究 |
| Study on radiosensitivity of AT fibroblasts line using apoptosis |
| 投稿时间：2006-04-24 |
| DOI： |
| 中文关键词: AT细胞 细胞辐射敏感性 3H-TdR掺入百分率 凋亡率 |
| 英文关键词:AT fibroblast Cell radiosensitivity Percentage of 3H-TdR incorporation Apoptosis rate |
| 基金项目:国家自然科学基金资助项目(30170288);安徽医科大学博士基金资助项目(XJ2004001) |
|
| 摘要点击次数: 3598 |
| 全文下载次数: 2290 |
| 中文摘要: |
| 目的 探讨AT细胞高辐射敏感性与细胞凋亡之间的联系。方法 以液体闪烁测量法测3H-TdR掺入百分率来观察60Coγ射线照射对AT5BIVA(AT)和GM0639(GM)细胞增殖的影响;实验于照射后0、24、48和72h,用细胞流式术动态检测AT和GM细胞的自发凋亡率及60Coγ射线照射诱发的凋亡率。结果 60Coγ射线0～6Gy照射后,AT和GM两种细胞3H-TdR掺入百分率均呈剂量依赖性降低,且AT细胞降低得比GM细胞快,两种细胞3H-TdR掺入百分率与受照射剂量间可拟合成指数方程:SAT=0.9718e-0.6855D(R2=0.9950)、SGM=1.0928e-0.3731D(R2=0.9777);AT细胞的自发凋亡率高于GM细胞;2～6Gy照后24h,两种细胞由辐射诱发的凋亡率均随照射剂量增大而升高,且在同一剂量点,前者凋亡率高于后者;2Gy照射后0～72h,AT和GM细胞的凋亡率均呈时间依赖性增加,且随照后时间延长两者间差异逐步增大,在72h达到显著水平。结论 AT细胞高辐射敏感性与其较高的自发及辐射诱发的凋亡密切相关。 |
| 英文摘要: |
| Objective To explore the relationship between the high radiosensitivity of AT5BIVA fibroblast line and apoptosis. Methods AT5BIVA(AT) fibroblasts and GM0639(GM) fibroblasts were exposed to 0-6 Gy 60Co γ-rays. The effects of 60Co γ-ray irradiation on the proliferation of AT and GM fibroblasts, expressed by percentage of 3H-TdR incorporation in DNA, were measured by liquid scintillation counter. The spontaneous and radiation-induced apoptosis rates were determined by flow cytometry at 0, 24, 48, and 72 h after exposure to 60Co γ-rays. Results The percentage of 3H-TdR incorporation in DNA in AT and GM fibroblasts decreased with radiation dose, and the 3H-TdR incorporation in AT fibroblasts decreased more quickly than that in GM fibroblasts. The relationships between 3H-TdR incorporation rate and dose were fitted as SAT =0.9718e-0.6855D (R2=0.9950) and SGM =1.0928e-0.3731D (R2=0.9777) for AT and GM fibroblasts, respectively. The spontaneous apoptosis rate in AT fibroblasts was higher than that in GM fibroblasts; the radiation-induced apoptosis rate in AT and GM fibroblasts gradually increased with dose at 24th hour after exposure to 2-6 Gy 60Co γ-rays, and radiation-induced apoptosis rate in the former was higher than that in the latter; the apoptosis rate in both AT and GM fibroblasts showed time-dependent increase during 0-72nd hour after exposure to 2 Gy 60Co γ-rays; the difference of the apoptosis rates between AT and GM fibroblasts gradually enlarged with dose and reached a significant level at 72nd hours after the exposure Conclusion The high radiosensitivity of AT fibroblasts was closely related to its spontaneous and radiation-induced apoptosis. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|