| 戴光复,金月英,田源,等.131I在细胞水平分布的实验研究[J].中华放射医学与防护杂志,2006,26(4):332-334.DAI Guang-fu,JIN Yue-ying,TIAN Yuan,et al.An experimental study of 131I microdistribution at cellular level[J].Chin J Radiol Med Prot,2006,26(4):332-334 |
| 131I在细胞水平分布的实验研究 |
| An experimental study of 131I microdistribution at cellular level |
| 投稿时间：2005-12-26 |
| DOI： |
| 中文关键词: 细胞水平 放射自显影 银颗粒 131I |
| 英文关键词:Cell level Microautoradiography Argent grain 131I |
| 基金项目:国家自然科学基金资助项目(10375091) |
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| 中文摘要: |
| 目的 用131I放射自显影技术观察其在细胞水平的分布,探讨放射性药物效果预测及评价。方法 采用放射自显影和冷冻切片技术,建立银颗粒密度与放射性药物强度的刻度曲线,确定放射性药物131I的微观分布,基于核素的微观分布数据,建立微剂量的剂量估算模式。结果 银颗粒密度与施入放射性药物比活度的相关系数为0.9963,刻度系数为1.59×10-4Bq。结论 131I在细胞水平的分布是不均匀的,银颗粒多数是分布在细胞浆中。因此在计算细胞水平的剂量时应考虑到其分布的不均匀性。 |
| 英文摘要: |
| Objective Traditional MIRD method for dose assessment in nuclear medicine assumes that radionuclide is homogeneously distributed in organs, the dose to cell and cell nucleus is presented by the mean dose to the organ. However, this assumption is hardly satisfication because of various factors, such as nuclide and its carrier, organ/cell and so on. Understanding micro-dose (i.e., doses at cell, meta-cell and molecule levels) distribution of radionuclide is an essential element for stipulating detailed plan for diagnosis and treatment in clinical nuclear medicine, as well as an important factor for predicting and assessing radio-pharmaceuticals effect. Methods In this work, the micro distribution of 131I is described using microautoradiogtraphy and frozen sectioning technique. Results To describe the relationship between argent grain density and nuclide radioactivity a regression curve was depicted, and the R value calculated was 0.9963. The scale coefficient of argent grain was 1.59×10-4 Bq. Conclusion The distribution of radiomedicament 131I at cellular level is nonuniform. |
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