| 唐古生,蔡建明,倪瑾,吴君,项莺松,崔建国,单鹄声,赵芳.反义STAT3寡核苷酸对B16细胞辐射敏感性的研究[J].中华放射医学与防护杂志,2006,26(4):324-327 |
| 反义STAT3寡核苷酸对B16细胞辐射敏感性的研究 |
| Study on radiosensitivity of B16 cells transfected by STAT3 antisense oligodeoxynucleotides |
| 投稿时间：2006-01-08 |
| DOI： |
| 中文关键词: 反义STAT3 B16 辐射敏感性 凋亡 肿瘤治疗 |
| 英文关键词:ASO-STAT3 B16 Radiosensitivity Apoptosis Tumor therapy |
| 基金项目:国家自然科学基金资助项目(30470415) |
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| 中文摘要: |
| 目的 探讨反义STAT3转染鼠恶性黑色素瘤B16细胞后,肿瘤细胞辐射敏感性的变化。方法 利用反义寡核苷酸转染B16细胞后,以不同剂量γ射线照射。通过CCK-8试剂盒检测细胞增殖变化,Hoechst33258染色对细胞凋亡作形态学上的观察。用Annexin V/PI复染,流式细胞仪检测细胞早期凋亡率的变化。结果 反义STAT3转染后加以γ射线照射,B16细胞的增殖相比于两者单独作用组受到明显抑制,细胞凋亡水平也增加。结论 反义STAT3寡核苷酸联合γ射线对B16细胞的增殖抑制和诱导凋亡作用明显增强,提高了鼠黑色素瘤B16细胞的辐射敏感性;表明阻断STAT3蛋白表达可能成为一种新的提高肿瘤辐射敏感性的有效手段。 |
| 英文摘要: |
| Objective To investigate the influence of STAT3 antisense oligodeoxynucleotides (ASO-STAT3) on radiosensitivity of B16 cells. Methods Oligodeoxynucleotides were tansfected into B16 cells with oligofectamine reagent. Cell survival was measured using CCK-8 assay. Apoptotic assays were performed using Hoechst33258 staining and Annexin V/PI with FACS analysis. Results Cell survival decreased significantly in those groups tansfected with ASO-STAT3 and irradiated with different dose of γ-rays. Compared with those cells treated with irradiation alone or AO-STAT3 transfection with subsequent exposure to radiation, fraction of early apoptosis increased in those cells treated with ASO-STAT3 transfection combined with irradiation. Conclusion These results showed that targeting ASO-STAT3 can enhance the therapeutic effect of γ-ray irradiation on B16 cells, which implied that STAT3 can act as a potential molecular target for tumor therapies, and also a potential molecular target for enhancement of radiosensitivity of radioresistant tumors. |
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