| 姚志军,陈永斌,郭国祯,孙晓莉.羟基卟啉对EMP照射损伤的防护作用[J].中华放射医学与防护杂志,2006,26(3):295-297 |
| 羟基卟啉对EMP照射损伤的防护作用 |
| Radioprotective effect of 5,10,15,20-tetrakis(4-hydroxyphenyl) porphyrin on endothelial cell of vein-304 |
| 投稿时间：2005-08-08 |
| DOI： |
| 中文关键词: 电磁脉冲 卟啉 人脐静脉内皮细胞 |
| 英文关键词:Electromagnetic pulse Porphyrin ECV cells |
| 基金项目:国家自然科学基金资助项目(50372084) |
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| 摘要点击次数: 2019 |
| 全文下载次数: 1847 |
| 中文摘要: |
| 目的 探讨meso-四(4-羟基苯基)卟啉[5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin]对电磁脉冲(EMP)照射离体人脐静脉内皮细胞的防护作用。方法 ①用MTT法进行meso-四卟啉对人脐静脉内皮细胞的毒性试验,找出最佳用药浓度,然后依此浓度作为防护药物浓度;②用化学发光法检测卟啉对人脐静脉内皮细胞在不同场强EMP照射下的防护效果。实验结果采用方差分析和独立样本t检验分析。结果 ①meso-四(4-羟基苯基)卟啉对人脐静脉内皮细胞的最佳用药浓度为0.01μg/ml;②经过EMP照射后,用药组化学发光强度(第1峰值和5s面积)明显低于对照组。结论 0.01μg/ml的meso-四(4-羟基苯基)卟啉对EMP具有一定的辐射防护作用。 |
| 英文摘要: |
| Objective To study the protective effect of 5,10,15,20-tetrakis (4hydroxyphenyl) porphyrin (THP) on endothelial cell of vein-304 (ECV-304) exposed to electromagnetic pulse (EMP). Methods MTT method was used to determine the optimal concentration of THP toxicity reaction for ECV-304 cells, and then used this concentration in the protection test. Chemiluminescence was used to measure the radioprotective effect of THP on ECV-304 cells exposed to varying intensity of EMP at 100,200, or 400 kV/m. ANOVA and independent samples t-test were used to analyze the data. Results The optimal concentration of THP for ECV-304 cells was estimated to be 0.01 μg/ml.The chemiluminescence intensities of ECV-304 cells administered with THP were statistically significantly lower than those in the control group. Conclusion This study suggested that 5,10,15,20-tetrakis (4hydroxyphenyl) porphyrin at 0.01 μg/ml can exert radioprotective effect of certain extent. |
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