| 张遵真,张勤,吴媚,李娜,衡正昌.60Coγ射线对hOGG1低表达细胞株放射敏感性的影响[J].中华放射医学与防护杂志,2006,26(3):238-241 |
| 60Coγ射线对hOGG1低表达细胞株放射敏感性的影响 |
| Effects of 60Co γ rays on radiosensitivity of down-regulated cell strain hOGG1 expressed genes |
| 投稿时间：2005-04-29 |
| DOI： |
| 中文关键词: hOGG1 放射敏感性 DNA损伤与修复 细胞周期 |
| 英文关键词:hOGG1 Radiosensitivity DNA damage and repair Cell cycle |
| 基金项目:国家自然科学基金(30571535);高等学校博士学科点专项科研基金资助项目(20040610082) |
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| 中文摘要: |
| 目的 研究60Coγ射线对DNA碱基切除修复基因hOGG1低表达细胞株放射敏感性的影响。方法 以A549细胞和通过稳定转染hOGG1核酶而获得的hOGG1低表达的A549-R细胞为研究对象,用MTT法测定不同剂量γ射线照射后两种细胞的存活率;单细胞凝胶电泳检测60Coγ射线处理后两种细胞DNA损伤与修复的差异;流式细胞术检测照射后两种细胞的周期分布、凋亡率和细胞增殖指数。结果 照射后两种细胞的存活率均随照射剂量的增加而下降,但A549-R细胞组的存活率显著低于相同剂量组的A549细胞(P<0.05);所设剂量均可诱导细胞DNA损伤,但DNA迁移长度和彗星细胞率在两种细胞间差异无统计学意义(P>0.05);损伤后的修复在两种细胞间差异有统计学意义(P<0.05),A549-R细胞的修复能力远远低于A549细胞。流式细胞术检测结果表明:照射后两种细胞都表现为G0/G1期细胞阻滞,细胞凋亡随照射剂量的增加而增加,细胞增殖指数随照射剂量的增加而降低,这些变化均以A549-R细胞更为明显。结论 hOGG1低表达使得细胞DNA修复能力降低,细胞周期阻滞于G0/G1期、细胞增殖减慢、凋亡增加、存活率下降,从而使细胞对60Coγ射线的放射敏感性增强。 |
| 英文摘要: |
| Objective To investigate the effect of 60Co γ rays on radiosensitivity of down-regulated cell strain hOGG1 expressed genes. Methods Human lung adenocarcinoma A549 cells and A549-R cells into which ribozyme genes (which inhibited the hOGG1 mRNA expression) were transfected were study materials. The cell survival rate after irradiation with different doses of 60Co γ rays was measured by MTT test. DNA damage and its repair were measured by single cell gel electrophoresis assay (SCGE). Flow cytometry was used to determine the apoptotic cell population, cell cycle distribution and cell proliferation index. Results The cell survival rate after irradiation was lower in the A549-R cells than that in the A549 cells. DNA damage induced by 2, 4, 8 or 12 Gy of 60Co γ rays was nearly the same in the two cell strains. The DNA repair showed a big difference in the two cell strains. The repair capability in the A549-R cells was significantly lower than that in the A549 cells. The percentages of A549-R cells in G0/G1 phase were increased with the gamma irradiation. Additionally, the percentages of cells in S and G2/M phase were significantly decreased in the two cell strains. The apoptosis percentages of the A549-R cells were obviously higher than those of the A549 cells when irradiated with the same dose of gamma rays. Both cell strains showed that the G0/G1 arrest, and cell proliferation index was decreased. Conclusion hOGG1 ribozyme increased the sensitivity to 60Co γ rays in the A549 cells. |
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