| 邓均,周桃莉,张春雷,郑峻松,李招权,黄辉,陈晓莉,程平.NO提高γ射线对L1210细胞损伤效应及其机制的研究[J].中华放射医学与防护杂志,2006,26(3):233-235 |
| NO提高γ射线对L1210细胞损伤效应及其机制的研究 |
| Study on nitric oxide enhancement effect of radiation damage to L1210 cells |
| 投稿时间：2005-06-29 |
| DOI： |
| 中文关键词: 一氧化氮 细胞凋亡 L1210 |
| 英文关键词:NO Apoptosis L1210 |
| 基金项目: |
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| 摘要点击次数: 2380 |
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| 中文摘要: |
| 目的 探讨一氧化氮(NO)是否提高γ射线对L1210细胞的损伤效应及其机制。方法 将L1210细胞加入隔离培养器内与3T3细胞共培养,收集经γ射线照射后12、24、48和72h各组L1210细胞,台盼蓝染色观察直接损伤作用,TUNNEL法观察诱导细胞凋亡的情况,流式细胞术检测细胞周期。结果 γ射线照射后质粒转染组L1210细胞台盼蓝拒染率下降最快,12h后与空载体组比较差异有统计学意义(P<0.05),加DEVD-CHO后能够提高台盼蓝拒染率,24h后有显著的差异(P<0.05);γ射线照射后质粒转染组细胞凋亡率24h开始明显升高,与空载体组比较差异有统计学意义(P<0.05),加DEVD-CHO后细胞凋亡率下降,24h开始与单独质粒转染组比较差异有统计学意义(P<0.05);射线照射后质粒转染组细胞G1期快速上升,4h与空载体组差异有统计学意义(P<0.05),加DEVD-CHO后G1期上升较单纯质粒转染组慢,4h两组差异有统计学意义(P<0.01)。结论 NO可增强γ射线对L1210细胞的直接损伤作用,出现细胞的G1阻滞,促进细胞凋亡。Caspase-3的活化参与上述作用。 |
| 英文摘要: |
| Objective To study whether nitric oxide(NO) could enhance the damage effect of radiation on L1210 cells in vitro. Methods L1210 cells co-cultured with different transfected 3T3 cells. The cells were divided into three groups, iPT group, PT group, and iPT plus DEVD-CHO group. The direct effect of NO and NO-induced apoptosis of L1210 cells in different groups at 12,24,48 and 72 h were detected by trypan blue rejecting dyeing (TRD) and TUNNEL, respectively. The cell cycles were detected by flow cytometry. Results The rate of TRD of L1210 cells during the time period of 12-72 h since irradiation was significantly lower in iPT group than that in PT group (P< 0.05). There was also significant difference between iPT group and iPT plus DEVD-CHO group during the time period of 24-72 h since irradiation (P<0.05). The apoptosis of L1210 cells during the time period of 24-72 h since irradiation in iPT group was significantly higher than that in PT group (P<0.05). There was also significant difference between iPT group and iPT plus DEVD-CHO group during the time period of 24-72 h since irradiation (P<0.05). L1210 cells at G1 phase at 4 h since irradiation increased more quickly in iPT group but declined more slowly during the time period of 24-72 h since irradiation than that in PT group (P<0.05). In iPT plus DEVD-CHO group,L1210 cells at G1 phase increased more slowly at 4 h than that in iPT group but declined more quickly during the time period of 24-72 h since irradiation(P<0.05). Conclusion NO can enhance the damage of radiation to L1210 cells through direct damage to L1210 cells; enhancement of apoptosis and blockage of G1 phase are dependent on Caspase-3 activation. |
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