| 杨岚,高瑛,田琼.血小板第4因子对放射损伤小鼠骨髓基质细胞周期的影响[J].中华放射医学与防护杂志,2006,26(3):226-228.YANG Lan,GAO Ying,TIAN Qiong.The effect of platelet factor 4 on cell cycle of bone marrow stromal cells in radiated mice[J].Chin J Radiol Med Prot,2006,26(3):226-228 |
| 血小板第4因子对放射损伤小鼠骨髓基质细胞周期的影响 |
| The effect of platelet factor 4 on cell cycle of bone marrow stromal cells in radiated mice |
| 投稿时间：2005-07-09 |
| DOI： |
| 中文关键词: 骨髓基质细胞 血小板第4因子 放射损伤 造血重建 |
| 英文关键词:Bone marrow stromal cell Platelet factor 4 Radiated injury Hematopoiesis recovery |
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| 中文摘要: |
| 目的 探讨血小板第4因子(plateletfactor4,PF4)对5.0Gyγ射线全身照射小鼠的骨髓基质细胞(bonemarrowstromalcells,BMSCs)的保护作用,进一步探讨PF4对造血的辐射防护机制。方法 30只雄性小鼠随机分为3组:①放射组,②PF4保护组,③对照组。小鼠照射前分别于26和20h腹腔内注射PF4,每次剂量50μg/kg。于照射后3d取骨髓细胞体外培养,分别计数培养后3、7和14d的骨髓基质细胞集落(CFU-F);在培养后10d流式细胞仪检测细胞周期。结果 3组中,照射组3d的CFU-F数量与PF4保护组差异无统计学意义,7和14d的CFU-F数量PF4保护组较照射组明显增加。流式细胞仪检测结果表明3组中照射组G0+G1期细胞明显高于其余两组,S,G2+M期细胞明显低于其余两组。结论 PF4对照射小鼠的骨髓基质细胞有保护作用,促进造血重建。 |
| 英文摘要: |
| Objective To investigate the protective effects of platelet factor 4(PF4) on bone marrow stromal cells (BMSCs)in the irradiated mice and explore its mechanism. Methods Thirty male mice were randomly divided into three groups; mice in one of the three groups were exposed to whole-body irradiation of 5.0 Gy 60Co gamma rays. The mice were intraperitoneally injected with PF4 (50 μg/kg) at 26 h and 20 h before the irradiation. The CFU-F was counted at 3 d,7 d, and 14 d after the cell culture, respectively. Cell cycle of BMSCs cultured in vitro after irradiation, were analyzed by FACS at 10 d since the culture. Results Compared with the group protected by PF4,the difference of number of CFU-F at 3 d in irradiation group was not statistically significant. But the difference at 7 d or 14 d was statistically significant. The cell percentage of G0+G1 phase in the irradiation group increased, while the percentage of S phase and G2+M phase decreased. Conclusion PF4 could protect BMSC in the irradiated mice and promote recovery of hematopoiesis. |
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