| 刘建香,阮健磊,苏旭,李文建.12C6+离子诱导TNFα基因在SMMC-7721细胞中的表达[J].中华放射医学与防护杂志,2006,26(3):224-226 |
| 12C6+离子诱导TNFα基因在SMMC-7721细胞中的表达 |
| Overexpression of TNFα gene in SMMC-7721 cells irradiated with 12C6+ ion |
| 投稿时间：2005-06-27 |
| DOI： |
| 中文关键词: 12C6+离子 人肝癌SMMC-7721细胞 TNFα表达 |
| 英文关键词:Carbon ion Human liver cancer cell line SMMC-7721 TNFα expression |
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| 中文摘要: |
| 目的 用Polyfect转染试剂将重组质粒pEgr-TNFα转染人肝癌细胞SMMC-7721,研究该重组质粒经12C6+离子作用后在细胞中的表达。方法 用ELISA方法检测TNFα的蛋白表达,用RT-PCR方法检测TNFα转录水平的变化。结果 转染pEgr-TNFα后在SMMC-7721细胞中TNFα的蛋白表达量为2.06ng/ml,2Gy12C6+离子照射后,表达量增高为2.11ng/ml,明显高于未转染组和转染空载体组;RNA转录水平也明显增高,2Gy12C6+离子照射后增高更明显。结论 12C6+离子联合重组质粒pEgr-TNFα,在被转染的SMMC-7721细胞中表达量明显增高。 |
| 英文摘要: |
| Objective To investigate the expression of pEgr-TNFα in SMMC-7721 cells, which were transfected by polyfect transfection reagent and irradiated with carbon ion 12C6+ ion. Methods The protein expression of TNFα was measured with ELISA, its transcription level was determined by RT-PCR. Results The expression of TNFα was measured to be 2.06 ng/ml when transfected by pEgr-TNFα, and increased to 2.11 ng/ml with irradiation of 2 Gy 12C6+, which was significantly higher than that of the control group or the vector group. The transcription level of TNFα was increased with the transfection; more significant higher level was observed with irradiation of 2 Gy 12C6+. Conclusion Carbon ions combined with pEgr-TNFα can significantly increase TNFα expression level in SMMC-7721 cells. |
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