中华放射医学与防护杂志

刘建香,阮健磊,苏旭,等.¹²C⁶⁺离子诱导TNFα基因在SMMC-7721细胞中的表达[J].中华放射医学与防护杂志,2006,26(3):224-226.LIU Jian-xiang,RUAN Jian-lei,SU Xu,et al.Overexpression of TNFα gene in SMMC-7721 cells irradiated with ¹²C⁶⁺ ion[J].Chin J Radiol Med Prot,2006,26(3):224-226

¹²C⁶⁺离子诱导TNFα基因在SMMC-7721细胞中的表达

Overexpression of TNFα gene in SMMC-7721 cells irradiated with ¹²C⁶⁺ ion

投稿时间：2005-06-27

DOI：

中文关键词: ¹²C⁶⁺离子人肝癌SMMC-7721细胞 TNFα表达

英文关键词:Carbon ion Human liver cancer cell line SMMC-7721 TNFα expression

基金项目:

作者	单位	E-mail
刘建香	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所	suxu@nirp.cn
阮健磊	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
苏旭	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
李文建	中国科学院近代物理研究所

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中文摘要:

目的用Polyfect转染试剂将重组质粒pEgr-TNFα转染人肝癌细胞SMMC-7721,研究该重组质粒经¹²C⁶⁺离子作用后在细胞中的表达。方法用ELISA方法检测TNFα的蛋白表达,用RT-PCR方法检测TNFα转录水平的变化。结果转染pEgr-TNFα后在SMMC-7721细胞中TNFα的蛋白表达量为2.06ng/ml,2Gy¹²C⁶⁺离子照射后,表达量增高为2.11ng/ml,明显高于未转染组和转染空载体组;RNA转录水平也明显增高,2Gy¹²C⁶⁺离子照射后增高更明显。结论 ¹²C⁶⁺离子联合重组质粒pEgr-TNFα,在被转染的SMMC-7721细胞中表达量明显增高。

英文摘要:

Objective To investigate the expression of pEgr-TNFα in SMMC-7721 cells, which were transfected by polyfect transfection reagent and irradiated with carbon ion ¹²C⁶⁺ ion. Methods The protein expression of TNFα was measured with ELISA, its transcription level was determined by RT-PCR. Results The expression of TNFα was measured to be 2.06 ng/ml when transfected by pEgr-TNFα, and increased to 2.11 ng/ml with irradiation of 2 Gy ¹²C⁶⁺, which was significantly higher than that of the control group or the vector group. The transcription level of TNFα was increased with the transfection; more significant higher level was observed with irradiation of 2 Gy ¹²C⁶⁺. Conclusion Carbon ions combined with pEgr-TNFα can significantly increase TNFα expression level in SMMC-7721 cells.

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