| 耿传营,向青,徐梅,等.太空环境对黑色素瘤B16细胞生物学特性的影响[J].中华放射医学与防护杂志,2006,26(3):207-209.GENG Chuan-ying,XIANG Qing,XU Mei,et al.Effects of space environment on biological characteristics of melanoma B16 cells[J].Chin J Radiol Med Prot,2006,26(3):207-209 |
| 太空环境对黑色素瘤B16细胞生物学特性的影响 |
| Effects of space environment on biological characteristics of melanoma B16 cells |
| 投稿时间：2005-11-07 |
| DOI： |
| 中文关键词: 太空环境 黑素瘤 B16 诱变 分化 |
| 英文关键词:Space environments Melanoma B16 Mutagenesis Differentiation |
| 基金项目:国家自然科学基金资助项目(30371324);国家科技部“973”前期资助项目[2003(CA04200)] |
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| 中文摘要: |
| 目的 初步研究太空环境对黑素瘤B16细胞生物学特性的影响。方法 将B16细胞搭载于第20颗返回式卫星,返地后回收细胞,单克隆化,获得110株太空B16细胞,按顺序编号。从中选取10株的太空B16单克隆细胞株,利用光镜观察其形态变化,MTT方法检测细胞增殖曲线,FCM测定细胞DNA含量,观察太空诱变细胞周期分布。结果 与对照细胞相比,太空B16细胞形态改变,有些细胞表面可见其分泌的黑色素颗粒;细胞增殖速率呈多向性变异;太空B16细胞G1期延长,S期缩短。结论 太空环境的复合因素可诱导B16细胞生物学特性发生变化。 |
| 英文摘要: |
| Objective To examine the effects of space environment on biological characteristics of melanoma B16 Cells. Methods B16 cells were carried to the space (in orbit for 8 days, circle the earth 286 times) by the 20th Chinese recoverable satellite,and then harvested and monocloned.110 strains of space B16 cells were obtained in total.Ten strains of space B16 cells were selected and its morphological changes were examined with the phasecontrast microscope.Flow cytometry and MTT assay were carried out to evaluate the cell cycle and cell viability. Results Morphological changes were observed in the space cells, and melainin granules on the surface in some cells. It was demonstrated by MTT assay that space cells viability varied muti-directionally. It was showed by flow cytometry analysis that G1 phase of space cells was prolonged, S phase shortened. Conclusion Space environment may change the biological characteristics of melanoma B16 cells. |
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