中华放射医学与防护杂志

孙丽,宋良文,刘涛,等.中国人γ-IFN对放射性肺纤维化的防治作用[J].中华放射医学与防护杂志,2006,26(2):144-147.SUN Li,SONG Liang-wen,LIU Tao,et al.Preventive effect of interferon-γ of Chinese genotype on radiation pulmonary fibrosis[J].Chin J Radiol Med Prot,2006,26(2):144-147

中国人γ-IFN对放射性肺纤维化的防治作用

Preventive effect of interferon-γ of Chinese genotype on radiation pulmonary fibrosis

投稿时间：2005-04-09

DOI：

中文关键词: γ-干扰素 Ⅳ型胶原人肺成纤维细胞

英文关键词:Interferon-γ Collagen Ⅳ Human lung fibroblast

基金项目:

作者	单位	E-mail
孙丽	100850, 北京放射医学研究所	lsong1@yahoo.com
宋良文	100850, 北京放射医学研究所
刘涛	100850, 北京放射医学研究所
张勇	100850, 北京放射医学研究所
尹纪业	100850, 北京放射医学研究所
刁瑞英	100850, 北京放射医学研究所

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中文摘要:

目的研究中国人γ干扰素对放射诱导的人肺成纤维细胞(HLF)异常增殖的抑制作用,探讨其对放射性肺纤维化的防治作用及机理。方法 MTT法检测细胞增殖,免疫细胞化学(SP)法观察成纤维细胞αSMA的表达及ColⅣ的合成。结果 ①⁶⁰Coγ射线能够诱导HLF的异常增殖,γIFN呈浓度依赖性抑制照射所致的HLF的异常增殖;②照射后HLF胞浆内有明显的αSMA的表达,表明照射可以诱导FB向MFB转化;③照射后ColⅣ的表达增高,照射前给予γIFN,ColⅣ的表达相对单纯照射组明显降低。结论 ⁶⁰Coγ射线通过直接促进HLF增殖、诱导FB向MFB转化和促进ColⅣ的分泌来诱导肺纤维化的发生;γ干扰素通过抑制HLF的异常增殖和ColⅣ的分泌,对放射性肺纤维化具有防治作用。

英文摘要:

Objective To investigate the inhibitive effect of Interferon-γ (γ-IFN) of Chinese genotype on proliferation of human lung fibroblast (HLF) induced by radiation, and examine its possible mechanism and role in prevention and treatment of radiation pulmonary fibrosis. Methods The proliferation of HLF was determined by MTT assay, the expression of γ-smooth muscle actin (α-SMA) and the synthesis of collagen type IV by immunocytochemistry assays. Results Chinese α-IFN was observed to inhibit, proportionally to irradiation dose, the proliferation of HLF induced by ⁶⁰Co γ-irradiation. The expression of α-SMA was remarkable in cytoplasmic matric after the irradiation, suggesting that irradiation could induce the transformation from fibroblast (FB) to myofibroblast (MFB). The expression of collagen Ⅳ was increased with irradiation. Compared with the irradiated group, the expression of collagen IV was lower when γ-IFN was administered before being irradiated.Conclusion ⁶⁰Co γ irradiation can induce pulmonary fibrosis by promoting the proliferation of HLF, inducing the transformation from FB to MFB, and increasing the excreting of collagen type Ⅳ.γ-IFN can prevent pulmonary fibrosis by effectively inhibiting the abnormal proliferation of HLF and the excessive synthesis of collagen Ⅳ after irradiation.

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