| 陈俊,余志坚,肖明星,等.P糖蛋白对高能X射线照射后Cyt-c释放和caspase-3活性的影响[J].中华放射医学与防护杂志,2006,26(2):118-120.CHEN Jun,YU Zhi-jian,XIAO Ming-xing,et al.The inhibition effect of P-glycoprotein on cytochrome c release and caspase-3 activation by X-ray irradiation.[J].Chin J Radiol Med Prot,2006,26(2):118-120 |
| P糖蛋白对高能X射线照射后Cyt-c释放和caspase-3活性的影响 |
| The inhibition effect of P-glycoprotein on cytochrome c release and caspase-3 activation by X-ray irradiation. |
| 投稿时间：2005-03-15 |
| DOI： |
| 中文关键词: P糖蛋白 细胞色素c caspase-3 X射线 |
| 英文关键词:P-glycoprotein Cytochrome c caspase-3 X-ray |
| 基金项目:国家自然科学基金资助项目(30100045) |
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| 中文摘要: |
| 目的 探讨抗P gp单抗UIC2对X射线照射后不同时间肿瘤细胞凋亡、细胞内cytochromec(Cyt c)释放和caspase3活性的影响。方法 以抗P gp单抗UIC2抑制P gp功能,X射线照射P gp表达的乳腺癌耐药细胞MCF7Adr。运用流式细胞仪检测X射线照射后细胞凋亡、细胞内Cyt c和caspase3活性的变化。结果 X射线照射后6、12和24h应用UIC2抑制P gp功能组细胞凋亡显著增加(P<0.01),胞浆Cyt c的表达均明显高于对照组(P<0.05),胞内caspase3活性均显著高于对照组(P<0.01)。结论 抑制P gp功能,增加了Cyt c释放和caspase3活性,提示P gp对X射线照射后肿瘤细胞胞浆Cyt c释放和caspase3活性均有抑制作用。 |
| 英文摘要: |
| Objective To investigate the effects of P-glycoprotein (P-gp) on cytochrome c (Cyt c)release and caspase-3 activation in drug-resistant tumor cell after X-ray irradiation. Methods Anti-P-gp McAb UIC-2 was applied to block P-gp function. MCF-7/Adr, P-gp-overexpressed drug-resistant breast cancer cell line, was irradiated by X-rays. Flow cytometry was used to measure apoptosis, dynamic changes of mitochondrial cytochrome c release and caspase-3 activation at various time after X-ray irradiation. Results Both the expression of cyt c and the activation of caspase-3 in P-gp-blocked group were up-regulated significantly, compared with those in control group at 6 h, 12 h, and 24 h after X-ray irradiation, respectively. Conclusion The current study showed that inhibition of P-gp function can increase cytochrome c release and caspase-3 activation.It suggested that P-gp inhibits X-ray induced cytochrome c release and caspase-3 activation. ; |
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