中华放射医学与防护杂志

王西玲,章翔,曹锐峰,曹云新,梁景文,马福成,边杰芳.⁶⁰Coγ射线对大鼠星形胶质瘤细胞增殖抑制作用的机理研究[J].中华放射医学与防护杂志,2006,26(1):55-58

⁶⁰Coγ射线对大鼠星形胶质瘤细胞增殖抑制作用的机理研究

Research on inhibitory effect of ⁶⁰Co γ-irradiation on C6 glioma proliferation and the related molecular mechanism

投稿时间：2005-06-06

DOI：

英文关键词:Glioma Apoptosis Cell cycle Flow cytometry p53 p21

基金项目:

作者	单位
王西玲	710032 西安,第四军医大学西京医院全军神经外科研究所
章翔	710032 西安,第四军医大学西京医院全军神经外科研究所
曹锐峰	710032 西安,第四军医大学西京医院全军神经外科研究所
曹云新	710032 西安,第四军医大学西京医院基础部医学免疫学教研室
梁景文	710032 西安,第四军医大学西京医院全军神经外科研究所
马福成	710032 西安,第四军医大学西京医院西京医院病理科
边杰芳	710032 西安,第四军医大学西京医院血管内分泌科

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中文摘要:

目的研究⁶⁰Coγ射线对大鼠星形胶质瘤细胞的增殖抑制作用的机制。方法 ⁶⁰Coγ射线单次照射,分为对照组、4、16和64Gy组。四甲基偶氮唑蓝(MTT)法测定细胞生长曲线。照射后48h采用流式细胞仪检测细胞凋亡和细胞周期分布,免疫组化法检测P53及P21蛋白的表达。结果 16和64Gy组C6细胞出现明显的增殖抑制。随着吸收剂量的增大,凋亡比例显著增加(P<0.01),G₀/G₁期的细胞比例增加,S期的细胞比例降低。野生型p53与p21随着照射剂量增加,表达增强。P53与P21蛋白表达强度呈正相关,相关系数斜率为0.889。结论 γ射线对大鼠星形胶质瘤细胞系C6细胞的增殖抑制通过诱导细胞凋亡和G₁期阻滞实现,G₁期阻滞的分子调控通路可能为p53-p21通路。

英文摘要:

Objective The purpose of this study was to observe the effect of ⁶⁰Co γ-irradiation on C6 rat astrocytoma cells and investigate the possible mechanism.Methods Cultured C6 cells were divided into 4 groups to receive different doses of γ-irradiation: control group, 4 Gy group,16 Gy group,and 64 Gy group.After irradiation the growth curve of cells was made by the methyl thiazolyl tetrazolium（MTT） assay.Flow cytometry was utilized to quantify the percentage of cell apoptosis and the cell cycle distribution 48 h after treatment.P53 and P21 expressions in the cells before and after treatment were analyzed by immunocytochemistry.Results Significant inhibition of proliferation was found in cells of the 16 Gy and 64 Gy groups.As the irradiation doses were raised,the apoptotic rates were significantly increased [3.1±0.5,16.7±0.6,27.3±0.4 and（36.7±0.7）% for the control,4 Gy,16 Gy and 64 Gy groups,respectively,P<0.01].Howerver,the necrotic rates of the four groups were not significantly different（P>0.05）.Meanwhile,as the doses of irradiation were raised,a significantly larger percentage of cells in G₀/G₁ phase and a smaller one in S phase were found.The expressions of P53 and P21 were both enhanced after irradiation and the enhancement was dose-dependent.Besides,the expressions of P53 and P21 were positively related and the correlation coefficient was 0.889. Conclusion The inhibition of proliferation of C6 cells after ⁶⁰Co γ-irradiation may be realized simultaneously by cell apoptosis,G₁ arrest and S delay.The p53-p21 pathway may be involved in this process.

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