| 邱小忠,朴英杰,余磊,等.γ射线照射诱导大鼠肝细胞线粒体自噬与再生的研究[J].中华放射医学与防护杂志,2006,26(1):50-52.QIU Xiao-zhong,PIAO Ying-jie,YU lei,et al.Study on γ-ray induced mitochondrial autophagy and regeneration of liver cells in rats[J].Chin J Radiol Med Prot,2006,26(1):50-52 |
| γ射线照射诱导大鼠肝细胞线粒体自噬与再生的研究 |
| Study on γ-ray induced mitochondrial autophagy and regeneration of liver cells in rats |
| 投稿时间：2005-07-08 |
| DOI： |
| 中文关键词: γ射线照射 线粒体自噬 凋亡 线粒体再生 |
| 英文关键词:γ-ray irradiation Mitochondrial autophagy Apoptosis Mitochondrial regeneration |
| 基金项目:广东省专项课题基金资助项目(A302020401);广东省科技攻关基金资助项目(2KM05503S);广州市科技攻关基金资助项目(2002Z1-E0037) |
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| 中文摘要: |
| 目的 探讨线粒体自噬在细胞损伤后再生中的作用。方法 在体外利用γ射线照射诱导大鼠肝细胞损伤,在亚细胞水平上观察肝细胞的损伤修复情况与线粒体自噬的关系,并利用分子生物学技术进行DNA凋亡片段的分析和线粒体RNA的RT-PCR分析。结果 受损线粒体清除过程存在线粒体自噬,随着修复过程的进行细胞DNA凋亡程度下降,细胞自噬现象减少,细胞凋亡和线粒体自噬同时存在于损伤细胞中,通过RT-PCR分析发现肝组织的损伤修复与线粒体自噬后的线粒体再生有关。结论 线粒体自噬可能为组织的损伤修复提供某种分子信号,促使新的线粒体再生。 |
| 英文摘要: |
| Objective To explore the role of mitochondrial autophagy in wound healing.Methods(Damages of) liver cells in rats after γ-ray irradiation with a total dose of 8 Gy were investigated.The relationship between mitochondrial autophagy and repair of liver cells was observed ultrastructurally.Apoptosis-related DNA fragment and mitochondrial RNA levels were analyzed.Results Most of the damaged mitochondria were removed through mitochondrial autophagy.Apoptosis-related DNA fragment analysis implied that,accompanied with reduction of mitochondrial autophagy,the amount of cellular apoptosis was decreased during wound healing.Wound healing was related to mitochondrial regeneration after mitochondrial autophagy.Conclusion Mitochondrial autophagy may release some molecular signals for wound healing so as to accelerate sufficiently the regeneration of newly formed mitochondria. |
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