| 周立霞,张明峰,杨天祝,檀国军.γ刀治疗大鼠脑胶质瘤后脑内和外周血TNF-α和IL-1β的表达[J].中华放射医学与防护杂志,2005,25(6):539-542 |
| γ刀治疗大鼠脑胶质瘤后脑内和外周血TNF-α和IL-1β的表达 |
| TNF-α and IL-1β expression changes in brain and peripheral blood after gamma knife treatment for rats with brain glioma |
| 投稿时间：2004-12-15 |
| DOI： |
| 中文关键词: 胶质瘤 γ刀 肿瘤坏死因子 白细胞介素-1β 原位杂交 |
| 英文关键词:Glioma Gamma knife Tumor necrosis factor-α Interleukin-1β(IL-1β) Hybridization in situ |
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| 中文摘要: |
| 目的 γ刀治疗脑胶质瘤后,观察脑内及外周血中TNF-α和IL-1β表达变化,探讨神经免疫调节在立体定向放射外科治疗中的作用。方法 成年雄性SD大鼠20只分4组,正常对照组(N组)、正常大鼠γ刀治疗组(NR组)、肿瘤对照组(T组)和肿瘤γ刀治疗组(TR组)。NR组和TR组大鼠于照射后14d,与各组大鼠进行脑组织TNF-α和IL-1βmRNA原位杂交和外周血TNF-α、IL-1β含量测定。结果 (1)原位杂交结果显示N和NR组大鼠脑内有少量TNF-α和IL-1β的mRNA表达,表现为大脑皮质、下丘脑核团等部位有少量弱阳性细胞散在分布。T组大鼠脑内阳性细胞面密度较N组和NR组升高(P<0.01),肿瘤区可见大量强阳性的胶质细胞和单核细胞浸润。TR组大鼠与T组相比,肿瘤区及周围脑区阳性细胞面密度明显增加(P<0.01)。(2)方差分析显示TR组和T组大鼠外周血TNF-α和IL-1β含量显著高于N组和NR组,TR组高于T组(P<0.01)。结论 脑胶质瘤大鼠γ刀治疗后,脑内TNF-α和IL-1βmRNA表达较治疗前增多,外周血TNF-α和IL-1β含量升高,这些变化可能与γ刀照射后胶质瘤大鼠体内免疫状态改变有关。 |
| 英文摘要: |
| Objective By observing the expressions of the TNF-α and IL-1β in the brain of rats with glioma after gamma knife(GK) therapy, to investigate the role of neuroimmunomodulation in the stereotaxic radiosurgery treatment. Methods A total of 20 normal adult male SD rats were divided into 4 groups: the normal control group (group N), normal rats with GK treatment group (group NR), tumor group (group T) and GK treatment for the tumor group (group TR). On day 14 after implantation of glioma cells, the rats in groups T and N were sacrificed and their brains were sampled, whereas the rats in groups TR and NR were undergone the GK irradiation with the same procedure mentioned above. On day 14 after irradiation, all of the rats were examined with in situ hybridization for TNF-α and IL-1β mRNA in the brain tissue, and the content of TNF-α in the peripheral blood was also determined. Results In groups N and NR, minimal levels of TNF-α and IL-1β mRNA could be detected in the brain, and a small amount of weak positive cells were dispersed in some areas such as the cerebral cortex, some hypothalamic nuclei, etc. The area density of the positive cells in the brain of the group T was higher than that of groups N and NR (P<0.01), and a large amount of infiltrating glial cells and monocyts were found in the tumour area. The area density of positive cells in the brain of the group TR was higher than that of group T(P<0.01); (2) ANONA test showed that the contents of TNF-α and IL-1β in the peripheral blood of group TR and group T were significantly higher than those in groups N and NR, and those of group TR were higher than those of group T(P<0.01). Conclusions After GK treatment, the levels of TNF-α and IL-1β mRNA increased in the brain of rats with glioma, and the TNF-α and IL-1β contents in the peripheral blood of the rats were also increased, suggesting that the changes might be related to the changes of the immune status induced by the gamma knife treatment. |
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