| 王春燕,张伟,苏旭.Okadaic acid诱导早熟凝集染色体测定辐射生物剂量[J].中华放射医学与防护杂志,2005,25(6):526-527 |
| Okadaic acid诱导早熟凝集染色体测定辐射生物剂量 |
| Okadaic acid for radiation dose estimation using drug-induced premature chromosome condensation |
| 投稿时间：2005-07-25 |
| DOI： |
| 中文关键词: 电离辐射 生物剂量 外周血淋巴细胞 早熟凝集染色体 |
| 英文关键词:Radiation Biodosimetry Peripheral blood lymphocytes Premature chromosomes condensedation |
| 基金项目:北京市自然科学基金资助项目(7032043号);科技部社会公益研究专项重点项目(2003DIA6N009) |
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| 中文摘要: |
| 目的 建立人外周血的早熟凝集染色体(prematurely condensed chromosomes,PCC)环与辐射剂量之间的剂量-效应关系曲线。方法 取健康成年人外周血,60Coγ射线分别以0、1、2、5、10、15、20和25Gy照射,吸收剂量率为1Gy/min,培养48h,终止培养前1h加入Okadaic acid诱导早熟凝集染色体,观察人外周血淋巴细胞PCC环与剂量效应的关系。结果 Okadaic acid诱导的人外周血淋巴细胞早熟凝集染色体环随着辐射剂量的增加而增加,但在20Gy以后达到饱和。结论 在20Gy剂量范围内,PCC环与辐射剂量具有良好的剂量-效应关系,与双着丝粒相比,它可作为大剂量受照情况下的生物剂量指示剂。 |
| 英文摘要: |
| Objective To establish simple biological method for high irradiation dose estimation using drug-induced prematurely condensd chromosomes(PCC) aberrations. Methods Peripheral blood was taken from healthy adults and irradiated by 0, 1, 2, 5, 10, 15, 20 and 25 Gy 60Co γ-rays. Then the blood was samples were cultured for 48 hrs. One hr before the end of culture, okadaic acid was added into culture medium to induce PCC rings,which were counted for each dose point. Results The yield of PCC rings was increased with the dose of radiation until 20 Gy. Within the range of 1 to 20 Gy, there was a good dose-response relationship between the yield of PCC rings and radiation dose. Conclusion Compared with the analysis of frequency of dicentrics, the yield of PCC rings could be a good biodosimetry indicator for estimation of high dose irradiation. |
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