| 陈剑,刘芬菊.超氧阴离子和过氧化氢对抗辐射菌增殖的影响[J].中华放射医学与防护杂志,2005,25(6):521-523 |
| 超氧阴离子和过氧化氢对抗辐射菌增殖的影响 |
| The effect of superoxide anion and hydrogen peroxide on the proliferation of Deinococcus radiodurans |
| 投稿时间：2005-01-04 |
| DOI： |
| 中文关键词: 抗辐射菌 活性氧 电离辐射 |
| 英文关键词:Deinococcus radiodurans Reactive oxygen species Ionizing radiation |
| 基金项目:国家自然科学基金资助项目(39900039) |
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| 中文摘要: |
| 目的 探讨不同浓度的超氧阴离子(O2.)和过氧化氢(H2O2)与抗辐射菌(Dr)增殖的关系。方法 利用细胞色素C还原法测定正常状态下和受不同剂量照射后Dr的O2.释放,加入还原型辅酶II(NADPH)氧化酶抑制剂DPI,观察在同样条件下Dr的O2.释放和增殖的变化。利用激光共聚焦显微镜测定受照后Dr内H2O2浓度变化。用3H-TdR掺入法测定上述条件下Dr的增殖。结果 Dr中O2.、H2O2的释放与其增殖有关,较小剂量(<1kGy)照射能刺激Dr增殖并释放O2.、H2O2,较大剂量下该效应被抑制。结论 适当浓度的O2.、H2O2对于Dr的增殖是必要的。 |
| 英文摘要: |
| Objective To study the relationship between the proliferation of Deinococcus radiodurans （Dr） and the concentrations of superoxide anion and hydrogen peroxide produced in irradiated Dr with different doses. Methods The release of O2. from Dr was determined with cytochrome C reduction method. Then diphenyleneiodonium （DPI）, the inhibitor of NADPH oxidase, was added into the culture medium. The concentration of H2O2 in Dr was measured with a laser confocal scanning microscope （LCSM）, and the release of O2. and Dr’s proliferation in the same circumstance with 3H-TdR incorporation method. Results The proliferation of Dr was related with the concentrations of O2. and H2O2. The radiation dose less than 1 kGy could stimulate proliferation of Dr, Whereas the proliferation was suppressed by higher dose of radiation. Conclusion Certain concentrations of O2.,H2O2 are essential to the proliferation of Deinococcus radioduras. |
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