中华放射医学与防护杂志

占宏伟,叶小娟,包承侃,等.Carbogen对肿瘤乏氧的改善以及在³²P间质内放疗中的作用[J].中华放射医学与防护杂志,2005,25(5):437-440.ZHAN Hong-wei,YE Xiao-juan,BAO Cheng-kan,et al.Study on improvement of tumor hypoxia and ³²P-colloid interstitial treatment effect by lasting inhalation of carbogen[J].Chin J Radiol Med Prot,2005,25(5):437-440

Carbogen对肿瘤乏氧的改善以及在³²P间质内放疗中的作用

Study on improvement of tumor hypoxia and ³²P-colloid interstitial treatment effect by lasting inhalation of carbogen

投稿时间：2005-01-16

DOI：

中文关键词: Carbogen 肿瘤乏氧 ³²P内放疗疗效

英文关键词:Carbogen Hypoxia Murine tumor ³²P-colloid interstitial treatment Effect

基金项目:

作者	单位
占宏伟	310009 杭州, 浙江大学医学院附属第二医院核医学科
叶小娟	310009 杭州, 浙江大学医学院附属第二医院核医学科
包承侃	310009 杭州, 浙江大学医学院附属第二医院核医学科
孙达	310009 杭州, 浙江大学医学院附属第二医院核医学科
何刚强	310009 杭州, 浙江大学医学院附属第二医院核医学科
张宏	310009 杭州, 浙江大学医学院附属第二医院核医学科

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中文摘要:

目的研究Carbogen长时间吸入的安全性,对肿瘤乏氧的改善以及对³²P肿瘤间质内放疗疗效的影响。方法 ①荷S180肿瘤小鼠10只,行吸入Carbogen前、后的^99mTHL91SPECT乏氧显像,比较两者TNT的变化。②正常小鼠20只,分为对照组和Carbogen吸入组,后者每日吸Carbogen2h,共24d。观察小鼠的日常活动、体重变化,及重要脏器病理学变化。③荷S180肿瘤小鼠40只,分为未治疗空气组、未治疗Carbogen组、³²P治疗空气组、³²P治疗Carbogen组,观察、对比各组小鼠肿瘤生长情况,检测抑瘤率和抑瘤曲线。结果 ①Carbogen吸入前后肿瘤^99mTHL91的TNT分别为1.872±0.391和1.354±0.189,差异有统计学意义(t=4.476,P<0.01);②未治疗空气组、未治疗Carbogen组小鼠间肿瘤生长速率无明显区别;³²P治疗空气组、³²P治疗Carbogen组小鼠在治疗后第12天抑瘤率分别为66.01%和69.43%,两者间无明显区别,在治疗后第24天,肿瘤体积分别为(2.728±0.469)和(2.237±0.603)cm³(t=2.128,P<0.05),肿瘤质量分别为(2.437±0.447)和(1.965±0.538)g(t=2.134,P<0.05)。③正常小鼠Carbogen长期吸入后日常活动、体重变化等与正常对照组间无明显区别,心、脑、肺、肝、脾、肾、小肠等脏器未见明显异常病理学改变。结论 Carbogen可以明显改善肿瘤乏氧状况,中长期吸入对小鼠毒副作用较小,在一定程度上可以增加³²P肿瘤间质内放疗的疗效。

英文摘要:

Objective To study the safety and improvement in tumor hypoxia and the ³²P-colloid interstitial treatment effect with lasting carbogen-inhaling in murine tumor models. Methods 1. The ^99mT-HL91 SPECT imaging was performed in 10 S180 tumor-bearing mice before and after carbogen inhalation for 2 hours; 2. Twenty normal BALB/c mice were divided into an air-group and a carbogen-group, in which carbogen was inhaled by mice 2 hours per day for 24 days. The activity body weight were observed, and pathologic examination of important organs was taken; 3. Forty mice bearing S180 tumor were divided into four groups: untreated air-inhaling group, untreated carbogen-group, ³²P-treated air-inhaling group, ³²P-treated carbogen-inhaling group. The volume of tumors and tumor inhibition curves were measured and compared in all mice,and the tumor inhibition ratio was calculated.Results 1.The T/NT of ^99mT-HL91 before and after carbogen inhalation was 1.872± 0.391 and 1.354± 0.189, respectively (t=4.476, P<0.01); 2. The tumor volume was not different between mice of untreated air-inhaling group and untreated carbogen-inhaling group; The tumor inhibition ratio was 66.01% and 69.43% respectively in the mice of ³²P-treated, air-inhaling group and ³²P-treated, carbogen-inhaling group on day 12 after ³²P-colloid interstitial treatment, whereas on day 24 the tumor volume was 2.728± 0.469 cm³ and (2.237± 0.603) cm³ (t= 2.128, P< 0.05) and the tumor mass was 2.437± 0.447 and (1.965± 0.538)g (t= 2.134, P< 0.05); 3. There were no differences in the activity, body weight between untreated air-inhaling mice and untreated carbogen-inhaling mice. No abnormal pathologic changes of important organs were found in untreated carbogen inhalation mice. Conclusion Long-term carbogen inhalation could significantly improve tumor hypoxia and ³²P-celloid interstitial treatment effect, and no toxicity was observed in mice.

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