| 张会来,王华庆,姚智,郝希山.人参皂甙Rg3促进同系小鼠外周血干细胞移植后免疫恢复研究[J].中华放射医学与防护杂志,2005,25(5):431-434 |
| 人参皂甙Rg3促进同系小鼠外周血干细胞移植后免疫恢复研究 |
| Study on effect of ginsenoside Rg3 on immunological recovery after peripheral blood stem cell transplantation in animal experiments |
| 投稿时间：2005-03-29 |
| DOI： |
| 中文关键词: 外周血干细胞移植 小鼠 免疫恢复 免疫治疗 |
| 英文关键词:Peripheral blood stem cell transplantation (PBSCT) Mice Immunological recovery Immune therapy |
| 基金项目:天津市科委自然科学基金资助项目(003119711) |
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| 中文摘要: |
| 目的 研究人参皂甙Rg3对小鼠外周血干细胞移植(PBSCT)后免疫恢复的促进作用。方法 首先建立PBSCT小鼠模型,然后将90只接受移植的小鼠随机分成6组,分别从移植后第15天起腹腔注射不同剂量Rg3、IL2及生理盐水。淋巴细胞转化实验观察T淋巴细胞功能的恢复;抗体形成实验(溶血素分光光度法)观察B淋巴细胞功能的恢复;杀伤实验观察NK细胞的功能恢复;流式细胞计数观察T亚群、B和NK细胞的恢复。结果 9mgkg+IL2联合治疗组显现出了良好的协同免疫增强作用,对细胞及体液免疫恢复的促进作用明显好于其他实验组及盐水组。NK细胞的恢复,各实验组之间无明显差异,但都要好于盐水组。结论 人参皂甙Rg3与IL2联合应用可明显加快移植后小鼠的免疫恢复过程,具有显著的免疫增强作用。两者的联合治疗要明显好于IL2或Rg3单药治疗效果。 |
| 英文摘要: |
| Objective To study the effect of ginsenoside Rg3 on immunological recovery in mice after peripheral blood stem cell transplantation (PBSCT). Methods A murine model of PBSCT was established. Ninety recipient mice were divided into 6 groups after transplantation: A, B, C, D, E, F groups, 15 mice each. They were intra-abdominally injected with Rg3 3 mg/kg, Rg3 6 mg/kg, Rg3 9 mg/kg,IL-2 and normal saline (NS), respectively. They were injected began from day 15 after PBSCT, daily for successive 15 dats per month, for a total of 3 months. The functional recovery of T and B lymphocytes was observed by the lymphocyte transformation test;and formation test of antibody, respectively, and the functional recovery of NK cell was observed by the killing test. The changes in number of T cell subpopulations, B cells and NK cells were observed by Flow cytometry (FCM). Results For the influence of Rg3 on cyto-immunity the action of 6 mg/kg and 9 mg/kg Rg3 was much better than that of NS (P<0.01) but not difference from that of IL-2. Only the combined group showed a good synergistic effect on immunological recovery. For the influence of Rg3 on humoral immunity, the effect of Rg3 3 mg/kg was stronger than NS but weaker than IL-2. The effect of Rg3 6 mg/kg and 9 mg/kg was similar to that of IL-2 (P>0.05). Only the combined group showed a good synergistic effect which was much better than that of other groups.For the influence on cytotoxic activity, the test groups were all better than NS group at the first month after PBSCT (P<0.05), but in the second month the cytotoxic activity of all test groups reduced gradually. However, in the third month the activity gradually recovered again, especially in Rg3 6 mg/kg, 9 mg/kg and 9 mg/kg+IL-2groups (P<0.01). Conclusion The combined use of ginsenoside Rg3 (high dose) and biological response modifier (BRM) improves greatly the immunological recovery of transplanted mice, and enhances greatly the immune function. The effect of combined therapy is much better than single IL-2 or Rg3 treatment. |
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