| 郝一文,李亚明,刘显智.辐照的血管内皮对造血干细胞迁徙的影响[J].中华放射医学与防护杂志,2005,25(5):428-431 |
| 辐照的血管内皮对造血干细胞迁徙的影响 |
| Study on the relation among protein kinase C activity,adhesion molecules expression of irradiated vascular endothelial cells and transmigration of haemopoietic stem cells |
| 投稿时间：2004-10-16 |
| DOI： |
| 中文关键词: γ射线 血管内皮细胞 蛋白激酶C 黏附分子 造血干细胞 迁徙 |
| 英文关键词:γ-rays Vascular endothelial cells Potein kinase C Adhesion molecules Migration Haemopoietic stem cells |
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| 中文摘要: |
| 目的 通过对小剂量γ射线辐照的血管内皮细胞的蛋白激酶C(PKC)活性、黏附分子表达及造血干细胞跨内皮迁徙率的测定,研究辐照的血管内皮对造血干细胞跨内皮迁徙能力的影响。方法 1～5Gyγ射线辐照人脐静脉内皮细胞(HUVECs);用[γ-32P]ATP掺入外源性底物测定PKC活性;用ELISA法测定黏附分子VCAM-1、PECAM-1、CD44的表达;用双室培养法测定造血干细胞跨HUVECs的迁徙能力。结果 3Gy以下γ射线辐照后的HUVECs膜PKC活性逐渐升高,浆PKC活性逐渐降低,尤以2、3Gy辐照的HUVECs变化最为显著(P<0.01),而高于3Gy辐照的HUVECs的膜、浆PKC活性呈下降趋势。3Gy以下辐照的HUVECs的VCAM1、PECAM1、CD44的表达量也显著升高(P<0.01)。造血干细胞通过γ射线辐照后的HUVECs的数量显著增加(P<0.01),但加入针对上述黏附分子的单克隆抗体后,能显著降低造血干细胞跨内皮能力。PKC活性与VCAM-1、PECAM-1、CD44的表达呈正相关关系。结论 适当小剂量的γ射线辐照血管内皮细胞,能活化其PKC、增加PKC活性,并进而上调黏附分子VCAM1、PECAM1、CD44的表达,有利于造血干细胞的跨内皮迁徙。 |
| 英文摘要: |
| Objective To study the relation among protein kinase C (PKC),adhesion molecules expression of vascular endothelial cells irradiated by small dose of γ-rays and transmigration of haemopoietic stem cells. Methods Human umbilical vein endothelial cells (HUVECs) were irradiated with 1-5 Gy γ-rays.The PKC activity of irradiated cells was measured by the incorporation of [γ-32P] ATP into exogenous substrate.The expression of adhesion molecules VCAM-1,PECAM-1, CD44 in irradiated HUVECs was detected by ELISA and transmigration of CD34++ haemopoietic stem cells across the irradiated HUVECs was determined by the transwell migration assay. Results PKC in HUVECs was activated by less than 3 Gy γ-irradiation,resulting in membranous PKC activity increasing and cytosolic PKC activity decreasing gradually.The dominant change occurred in HUVECs irradiated by 2-3 Gy γ-rays (P<0.01).There was a decreasing trend of membranous and cytosolic activity PKC in HUVECs irradiated by more than 3 Gy γ-rays.The expressions of VACM-1,PECAM-1 and CD44 in HUVECs irradiated by less than 3 Gy γ-rays were significantly increased compared with the results of the control group(P<0.01).The numbers of CD34++ haemopoietic stem cells migrating across the irradiated HUVECs were much higher than those in the control group (P<0.01),but monoclonal antibodies to VACM-1,PEACM-1,CD44 could depress the transmigration activity.There was a positive relation beween the PKC activity and the expression of VCAM-1,PECAM-1 and CD44 respectively. Conclusions Optimal small dose of γ-rays could activate PKC,enhance PKC activity of irradiated vascular endothelial cells,and up-regulate the adhesion molecules expression of VACM-1,PECAM-1 and CD44,resulting in promoting the transendothelial migration of haemopoietic stem cells. |
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