| 杨慧,李良,张顺英,等.99mTc-抗D-二聚体单抗对实验动物陈旧性血栓的放射免疫显像研究[J].中华放射医学与防护杂志,2005,25(5):420-422.YANG Hui,LI Liang,ZHANG Sun-ying,et al.Radioimmunoscintigraphy of experimental old thrombi in animals with 99mT-labeled anti-D-dimer monoclonal antibody[J].Chin J Radiol Med Prot,2005,25(5):420-422 |
| 99mTc-抗D-二聚体单抗对实验动物陈旧性血栓的放射免疫显像研究 |
| Radioimmunoscintigraphy of experimental old thrombi in animals with 99mT-labeled anti-D-dimer monoclonal antibody |
| 投稿时间：2004-10-25 |
| DOI： |
| 中文关键词: 99mT 抗D二聚体单克隆抗体 血栓 |
| 英文关键词:99mT Anti-D-dimer monoclonal antibody Thrombus |
| 基金项目:北京市教委基金资助项目(01KJ094) |
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| 中文摘要: |
| 目的 研究99mT标记的抗D二聚体单抗在陈旧性动脉血栓和静脉血栓靶向定位诊断中的应用。方法 采用机械损伤血管内膜的方法复制家兔股动脉血栓和股静脉血栓模型,血栓形成后3d将99mT标记的抗D二聚体单抗注入动物血管内,以放射免疫显像检测技术观察放射性核素在血流和血栓部位的分布。结果 血栓处放射性99mT的分布较健侧明显增多,而单纯钳夹血管组及正常对照组未见局部放射性物质的堆积。注入99mT标记的单抗后,动物未见不良反应,主要脏器未见病理改变。结论 99mT标记的抗D二聚体单抗可以作为血栓导向示踪剂,用于陈旧性动脉血栓和静脉血栓的定位诊断。 |
| 英文摘要: |
| Objective To evaluate the use of 99mT_labeled anti-D-dimer monoclonal antibody in detection of experimental old thrombus in rabbits. Methods 99mT-labeled anti-D-dimer monoclonal antibody was administelred into femoral vein and artery of the rabbits 3 days after formation of thrombi by mechanical injury of endothelium of these blood vessels. Radioactivity distribution specificity of 99mT-anti-D-dimer was shown by radionuclide imaging technique respectively at 5 min,10 min,30 min after the injection. In both intact and thrombosed animals. Results 99mT-anti-D-dimer was specifically concentrated in site of thrombus, but not concentrated in corresponding vessels of normal group rabbits, nor in the vessels having been closed by clamps of the simple clamping group. In addition, no abnormal reaction was resulted from the injection of 99mT-anti-D-dimer and no pathologic changes were found in main organs of rabbits. Conclusion The 99mT-labeled anti-D-dimer antibody will be one of potential targeting tools for localizational diagnosis of old thrombu in either artery or vein. |
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