| 涂序珉,雷苏文,王菊芳,张斌,张志兴,吕慧敏,李文建.用培养基介导法研究辐射旁效应及其机理[J].中华放射医学与防护杂志,2005,25(4):333-335 |
| 用培养基介导法研究辐射旁效应及其机理 |
| Study on bystander effect and associated mechanism mediated through culture medium |
| 投稿时间：2004-12-28 |
| DOI： |
| 中文关键词: 自然杀伤细胞活性 旁效应 活性氧 肿瘤细胞Yac-Ⅰ |
| 英文关键词:Activity of natural killer cells Bystander effect Reactive oxygen species Tumor cell Yac-Ⅰ |
| 基金项目:国家自然科学基金资助项目(10105012) |
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| 中文摘要: |
| 目的 通过受照射的细胞培养液培养靶细胞,并用二甲基亚砜(DMSO)对靶细胞预处理,检测其对自然杀伤(NK)细胞活性的影响,研究旁效应是否存在及其产生机理。方法 以健康雄性ICR小鼠脾NK细胞活性检测经过不同剂量照射的细胞培养液对小鼠淋巴瘤YacⅠ细胞的损伤程度,同时观察DMSO对该细胞的保护作用,即对旁效应的抑制作用。结果 应用不同剂量60Coγ射线照射的细胞培养液来培养YacⅠ细胞,引发该细胞不同程度的损伤,表现在YacⅠ细胞对小鼠脾NK细胞的敏感性增强(P<0.01);DMSO预处理细胞对旁效应有一定的抑制作用,特别在0.25和0.5Gy组,对细胞的保护作用明显。结论 60Coγ射线照射能诱发YacⅠ细胞的培养液介导的旁效应。活性氧(ROS)是诱发辐射旁效应的因素之一,清除ROS可部分抑制辐射旁效应。 |
| 英文摘要: |
| Objective To study the bystander effect and associated mechanism mediated through the irradiated cell culture medium. Methods Splenic natural killer (NK) cells were obtained from healthy male ICR strain mice. Culture medium irradiated with different doses of 60Coγ-rays was used for culturing Yac-Ⅰ lymphoma cells. The degree of injury of the latter by activated NK cells was observed. A part of the culture media were pretreated with 1% DMSO, a scavenger of reactive oxygen species(ROS),in order to investigate the possible mechanism of a radiation-induced bystander response. Results Severer injury was induced in Yac-Ⅰ cells cultured in the media pre-irradiated with different doses of γ-rays than that in Yac-Ⅰ cells cultured in unirradiated medium, as shown by increased sensitivity to murine splenic NK cells (P<0.01). Culturing Yac-Ⅰ cells in DMSO-pretreated medium considerably reduced the activation of NK cells, especially in 0.25 Gy and 0.5 Gy γ-irradiated media. Therefore, it can be expected that DMSO can partly suppress ROS-induced bystander effect. Conclusion The irradiated culture medium of Yac-Ⅰ cells can trigger bystander effect. ROS likely plays an important role in radiation-induced bystander effect that can be partly suppressed by pretreatment with DMSO. |
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