| 李杨,宋良文,王德文,等.γ射线对肺成纤维细胞中转录因子SP1,AP1和Smad3-Smad4活性的影响及意义[J].中华放射医学与防护杂志,2005,25(4):329-332,324.LI Yang,SONG Liang-wen,et al.Effect of γ-rays on transcriptional activity of SP1, AP1 and Smad3-Smad4 in pulmonary fibroblasts[J].Chin J Radiol Med Prot,2005,25(4):329-332,324 |
| γ射线对肺成纤维细胞中转录因子SP1,AP1和Smad3-Smad4活性的影响及意义 |
| Effect of γ-rays on transcriptional activity of SP1, AP1 and Smad3-Smad4 in pulmonary fibroblasts |
| 投稿时间：2004-10-30 |
| DOI： |
| 中文关键词: γ射线 转录因子 胶原 肺成纤维细胞 |
| 英文关键词:γ-rays Transcriptional factor Type Ⅰ collagen Lung fibroblast |
| 基金项目:国家自然科学基金资助项目(30170292) |
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| 中文摘要: |
| 目的 检测不同剂量γ射线照射对大鼠肺成纤维细胞(rat lung fibroblast,RLF)中转录因子SP1,AP1和Smad3-Smad4活性的影响并探讨其意义。方法 进行大鼠肺成纤维细胞的原代分离培养,应用3、5和8Gyγ射线照射,通过凝胶阻滞电泳(electrophoretic mobility shift assay,EMSA)、免疫印迹和免疫组织化学等方法检测照射后转录因子SP1,AP1和Smad3-Smad4活性的变化及Ⅰ型胶原和Ⅰ型组织纤溶酶原激活物抑制剂(type Ⅰ plasminogen activator inhibitor,PAI-Ⅰ)的表达变化。结果 正常RLF中AP1和Smad3-Smad4形成弱阻滞条带,SP1无阻滞条带形成,3、5和8Gyγ射线照射后,3种转录因子所形成的阻滞条带明显增强,PAI-Ⅰ和Ⅰ型胶原的表达增多。结论 一定剂量γ射线能促进大鼠肺成纤维细胞中转录因子SP1,AP1和Smad3-Smad4的活化,并进而促进PAI-Ⅰ和Ⅰ型胶原的表达。 |
| 英文摘要: |
| Objective To investigate the effects of γ rays on the transcriptional activity of SP1, AP1 and Smad3-Smad4 in pulmonary fibroblasts. Methods Rat pulmonary fibroblasts were separated,cultured and then irradiated with 3, 5 and 8 Gy 60Coγ-rays. The transcriptional activity of SP1, AP1 and Smad3-Smad4 and the expression of PAI-Ⅰ and type Ⅰcollagen were observed by means of EMSA, immunoblotting and immunohistochemistry. Results In normal fibroblasts, AP1, Smad3-Smad4 formed weak specific shift binding blot,but SP1 did not. After irradiated with 3, 5 and 8 Gy 60Coγ-rays, the transcriptional factors AP1, SP1 and Smad3-Smad4 were activated and the expression levels of type Ⅰcollagen and PAI-Ⅰ were increased. Conclusion A certain dose of γ-rays may promote the activation of transcriptional factors SP1, AP1 and Smad3-Smad4 and thus increase the expressions of PAI-Ⅰ and type Ⅰ collagen. |
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