| 戴光复,金月英,寇明英,苑淑渝,张良安.99mTc-DTPA细胞水平分布的实验研究[J].中华放射医学与防护杂志,2005,25(4):319-321 |
| 99mTc-DTPA细胞水平分布的实验研究 |
| An experimental study of microdistribution of 99mTc-DTPA at cellular level |
| 投稿时间：2005-02-16 |
| DOI： |
| 中文关键词: 细胞水平 放射自显影 放射性药物 银颗粒 99mTc-DTPA |
| 英文关键词:Cell level Microautoradiography Radiomedicament Argent grain 99mTc-DTPA |
| 基金项目:国家自然科学基金资助项目(10375091) |
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| 中文摘要: |
| 目的 核医学中传统的MIRD剂量估算方法是假设放射性药物在器官内均匀分布,用器官的平均剂量描述每个细胞及细胞核的剂量。基于核素的微观分布数据,建立微剂量的剂量估算模式,从而为核医学中诊疗计划的制定、放射药物效果和危害的预测及评价、分子核医学研究提供基础的微剂量估算及其分布研究的方法和基础数据。方法 采用了放射自显影术和冰冻切片技术,建立自显影银颗粒密度与放射性药物强度的刻度曲线,确定放射性药物99mTcDTPA的微观分布。结果 银颗粒密度与施入比活度的相关系数为0.9915,刻度系数为6.48×10-5Bq。细胞浆与细胞核的分布比为1.78。结论 放射性药物在细胞水平的分布是不均匀的,因此在计算细胞水平的剂量时应考虑到其分布的不均匀性。 |
| 英文摘要: |
| Objective Traditional MIRD method for dose assessment in nuclear medicine assumes that radionuclide is homogeneously distributed in organs, and the dose to cells and cell nucleus is presented by mean dose to the organ. This project will provide the basic method and data for planning diagnosis and treatment in nuclear medicine, for predicting and evaluating effect and risk of radio-pharmaceuticals, and for further research of microdosimetry at molecular level in nuclear medicine and biology. Method The article will describe the microdistribution of 99mTc-DTPA using microautoradiography and frozen sectioning technique. Results To describe the relationship between argent grain density and nuclide radioactivity a regression curve was depicted and the R value was calculated to be 0.9915. The grain density over cytoplasm was 1.78 times higher than that over nucleus in these cells. Conclusion The distribution of radiomedicament at cellular level is nonuniform.; |
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