| 王枫,艾毅钦,张铭.电离辐射对松果腺细胞功能的影响[J].中华放射医学与防护杂志,2005,25(3):240-242.WANG Feng,AI Yi-qin,ZHANG Ming.Effect of ionizing irradiation on function of pineal gland cells[J].Chin J Radiol Med Prot,2005,25(3):240-242 |
| 电离辐射对松果腺细胞功能的影响 |
| Effect of ionizing irradiation on function of pineal gland cells |
| 投稿时间：2004-06-01 |
| DOI： |
| 中文关键词: 松果腺 电离辐射 褪黑素 细胞凋亡 |
| 英文关键词:Pinealgland Ionizing radiation Melatonin Cell apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的 研究电离辐射对松果腺细胞凋亡、cAMP和褪黑素(MLT)含量的变化规律及特点。方法 采用流式细胞术检测松果腺细胞凋亡。采用放免分析法检测松果腺细胞中cAMP含量。采用高效液相色谱分析法检测松果腺细胞中MLT含量。结果 小鼠受0.5～6GyX射线全身照射12h,随剂量增加松果腺细胞凋亡百分率增加;照射后24h,随剂量增加松果腺细胞中cAMP含量降低。小鼠受1～6Gy照射后12h,随剂量增加松果腺细胞合成和分泌MLT功能减弱。结论 高剂量电离辐射对松果腺细胞功能有抑制作用。 |
| 英文摘要: |
| Objective To research the effects of ionizing radiation on apoptosis and pattern of changes in cAMP and melatonin (MLT) contents of pinealocytes. Methods The percentage of apoptosis of pinealocytes in mice was determined with flow cytometry. The contents of cAMP and MLT in pinealocytes were measured with radioimmunoassay and high preformance liquid chromatography, respectively. Results The percentage of apoptotic pinealocyte was observed in mice 12 hours after irradiation. The results showed that the percentage of cell apoptosis increased gradually after irradiation with 0.5-6.0 Gy X-rays. The content of cAMP in mouse pinealocytes reduced gradully after irradiation with 1.0-6.0 Gy, and was significantly lower after irradiation with 4.0-6.0 Gy than that in the control group (P<0.01). The content of MLT secreted by mouse pinealocytes decreased gradually 12 hours after irradiation with 1.0-6.0 Gy X-rays. Conclusion High dose ionizing radiation has inhibitory effect on the function of pinealocytes. |
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