中华放射医学与防护杂志

胡锴勋,孙琪云,刘丽辉,等.间充质干细胞治疗急性骨髓型放射病的实验研究[J].中华放射医学与防护杂志,2005,25(3):221-224.HU Kai-xun,SUN Qi-yun,LIU Li-hui,et al.Experimental study of MSCs in treatment of acute hematopoietic radiation injury[J].Chin J Radiol Med Prot,2005,25(3):221-224

间充质干细胞治疗急性骨髓型放射病的实验研究

Experimental study of MSCs in treatment of acute hematopoietic radiation injury

投稿时间：2004-07-05

DOI：

中文关键词: 急性骨髓型放射病骨髓间充质干细胞细胞周期凋亡

英文关键词:Acute hematopoietic radiation injury Mesenchymal stem cells Cell cycle Apoptosis

基金项目:国家“863”资助项目(2002AA216081)

作者	单位
胡锴勋	100039 北京, 军事医学科学院附属医院血液科
孙琪云	100039 北京, 军事医学科学院附属医院血液科
刘丽辉	100039 北京, 军事医学科学院附属医院血液科
范传波	100039 北京, 军事医学科学院附属医院血液科
黄雅静	100039 北京, 军事医学科学院附属医院血液科
郭梅	100039 北京, 军事医学科学院附属医院血液科
艾辉胜	100039 北京, 军事医学科学院附属医院血液科

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中文摘要:

目的研究骨髓间充质干细胞(MSCs)在治疗急性骨髓型放射病中的作用,并对其机理做初步的探索。方法 BALBc雌性小鼠分成MSCs组和照射对照组,MSCs组经5.5Gy⁶⁰Coγ射线照射,2h内尾静脉输注C57BL6雄性小鼠的MSCs,照射对照组照射后不作处理。观察各组外周血象变化、骨髓细胞凋亡、细胞增殖周期、骨髓病理变化、骨髓造血粒系祖细胞集落(CFUGM)以及基质细胞集落(CFU-F)计数。结果与对照组相比,MSCs组的三系血细胞下降慢,最低值抬高,并且造血恢复更迅速,尤其白细胞、血小板变化更明显。骨髓细胞凋亡率和P53蛋白表达量少于对照组。照后2d,MSCs组G₀/G₁期比值较对照组明显下降(P<0.01),G₂M期比值及S期计数较对照组显著升高(P<0.01)。照后4dMSCs组胸骨骨髓增生活跃,对照组增生减低,照后20dMS-Cs组新生造血灶多于对照。CFU-GM、CFUF计数在照后初期、极期、恢复期均高于对照。结论 MSCs对急性放射病治疗有良好的治疗作用,其机制可能与MSCs减少细胞凋亡、改善造血微环境、促进干细胞增殖分化有关。

英文摘要:

Objective To investigate the effect and mechanism of MSCs on hematopoietic reconstitution in irradated mice with 5.5 Gy ⁶⁰Coγ_rays. Methods BALB/c mice irradiated with 5.5 Gy ⁶⁰Coγ_rays were randomly divided into a control group and a MSCs_treated group.Peripheral leukocyte,platelet,erythrocyte counts,apoptosis and cell cycle of bone marrow cells (BMC),pathologic change of bone marrow and numbers of GM_CFU and F_CFU were observed. Results Compared to the control group three series of BMC in MSC_treated group decrease more slowly,but elevation of the nadir and recovery were more rapid.The apoptotic ratio and p53 expression in controls were superior to those in MSC_treated group.The ratio of G₀/G₁ was obviously dropped in the MSC_treated group (P<0.01); however,the ratio of G₂/M and the number of cells in S stage were higher in that group (P<0.01).The sternum marrow regenerated actively MSC treatment,while marked hypoplasia was seen in controls on day 4.More hematopoietic islands in the bone marrow were observed in MSC_treated group on day 20 and the CFU_GM and CFU_F counts were higher than those in controls. Conclusion (These results ) indicate that MSC injection may become a valuable strategy for acute hematopoietic injury.

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