| 方恒虎,曾桂英,任东青,金成,黄晓峰,郭鹞.脉冲电磁场对大鼠垂体超微结构的影响[J].中华放射医学与防护杂志,2005,25(2):175-177 |
| 脉冲电磁场对大鼠垂体超微结构的影响 |
| Study on ultrastructural changes of the pituitary gland in rats exposed to pulsed electromagnetic fields (PEMF) |
| 投稿时间：2005-01-18 |
| DOI： |
| 中文关键词: 脉冲电磁场 大鼠 垂体 超微结构 |
| 英文关键词:Pulsed electromagnetic fields (PEMF) Rat Pituitary gland Ultrastructure |
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| 中文摘要: |
| 目的 探讨脉冲电磁场(PEMF)对大鼠垂体前叶内分泌细胞超微结构的影响。方法 SD雄性大鼠30只,随机分为5组,每组6只。其中1组为对照组,其余4组为辐照组。以场强200kVm的PEMF对辐照组大鼠进行200次脉冲的全身辐照。于照后6、12、24和48h活杀动物,取出垂体,制作常规电镜标本,观察垂体前叶内分泌细胞超微结构的改变。结果 辐照后6h大鼠垂体前叶内分泌细胞超微结构即表现为变化,其中催乳素细胞及生长激素细胞变化明显,表现为线粒体肿胀、嵴断裂、包涵体形成,高尔基复合体扩张及散在的各级溶酶体;至照后48h变化呈逐渐加重趋势,包括线粒体空泡化及其内部髓鞘样结构形成,内质网高度扩张,大量的次级溶酶体出现,细胞核内异染色质边集等。结论 PEMF辐照可对大鼠垂体前叶内分泌细胞结构造成损伤,其主要受损细胞器为线粒体和内质网。 |
| 英文摘要: |
| Objective To observe the effects of exposure to PEMF on ultrastructure of pituitary gland of rats. Methods Altogether 30 male SD rats were randomly divided into five groups, one for sham and the others for PEMF exposure(200 kV/m, 200 times) pulses. After exposure, the rats were scacrificed and pituitary glands were taken out at different time points(6, 12, 24 and 48h). Ultrastructural changes of the prepared samples were observed under a transmission electron microscope. Results At 6 h after PEMF exposure, swollen mitochondria with cristae loss, dilatation of Golgi complex and diffused lysosomes were found in endocrine cells of pituitary gland, especially in lactotrophic cells and growth hormone_secreting cells. These changes were gradually aggravated, mitochondrial vacuolization and forming of myelin figures, distinct dilatation of endoplasmic reticulum, occurrence of numerous secondary lysosomes and gathering of heterochromatins under nuclear membranes could be observed at 48 h. Conclusions PEMF may damage endocrine cells in anterior lobe of pituitary gland of rats. Mitochondria and endoplasmic reticulum are the sensitive organelles to PEMF. |
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