| 于大海,王代友.血管内皮生长因子转染放疗组织的研究[J].中华放射医学与防护杂志,2005,25(2):132-134.YU Da-hai,WANG Dai-you.Research on revascularization of irradiated tissue by VEGF gene transfection[J].Chin J Radiol Med Prot,2005,25(2):132-134 |
| 血管内皮生长因子转染放疗组织的研究 |
| Research on revascularization of irradiated tissue by VEGF gene transfection |
| 投稿时间：2004-05-24 |
| DOI： |
| 中文关键词: 血管内皮生长因子 基因转染 放射损伤 |
| 英文关键词:Vascular endothelial growth factor Gene transfection Irradiated injury |
| 基金项目:国家自然科学基金资助项目(30060083) |
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| 中文摘要: |
| 目的 用血管内皮生长因子基因转染的方法恢复放疗损伤区组织血管生成能力。方法 以60Coγ射线照射大鼠右后肢,每次每只大鼠吸收剂量为2Gy,总吸收剂量为30Gy。照射结束后第1天,将重组质粒pcDNA3/VEGF165转染大鼠右后肢照射区肌肉组织细胞,每次每只大鼠转染质粒200μg,共治疗2次,间隔2周,治疗结束后行VEGF165mRNA、VEGF蛋白、血管染色体视学图像分析及血管超微结构观察等检查。结果 经重组质粒pcDNA3/VEGF165转染后,照射区组织VEGF165mRNA及VEGF蛋白均明显增高,微血管密度、平均截面积、平均管径均达到正常组织水平,血管超微结构基本恢复正常。结论 VEGF基因转染不仅有效地修复了组织血管放射损伤,而且还能恢复其血管生成能力,为临床应用提供了实验基础。 |
| 英文摘要: |
| Objective To resume the ability of revascularization of the irradiated tissue by VEGF gene transfection. Methods Each one of the mice was irradiated for 2 Gy every time and 30 Gy in total on the right leg. The irradiated muscular tissues of animal models were transfected with 200 μg pcDNA3/VEGF165 per animal on the right leg and another 200 μg two weeks later. After the gene therapy we determined VEGF165 mRNA,VEGF protein, vessel density, average diameter of vessels and average sectional aera. We also observed ultrastructure of the vessels at the same time. Results VEGF165 mRNA and VEGF protein were highly expressed in the irradiated tissue. Vessel density, average diameter and average sectional aera were also not different from those on the left leg, and the configuration and ultrastructure of vessels returned to normal. Conclusion VEGF gene therapy can resume the ability of revascularization of irradiated tissue, which provides the bases for its clinical application. |
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