| 何贵金,张宏,高沁怡,许书河,高红,姜维国,蒋涛,戴显伟,马凯.电离辐射诱导犬胆管成纤维细胞凋亡及防治胆管损伤后瘢痕增生的研究[J].中华放射医学与防护杂志,2005,25(2):118-120 |
| 电离辐射诱导犬胆管成纤维细胞凋亡及防治胆管损伤后瘢痕增生的研究 |
| The role of γ-ray-induced fibroblast apoptosis in inhibiting biliary duct hypertrophic scar formation in dogs |
| 投稿时间：2004-03-15 |
| DOI： |
| 中文关键词: 103Pd支架 γ射线 细胞凋亡 |
| 英文关键词:Apoptosis γ-radiation 103Pd radioactive stent |
| 基金项目: |
|
| 摘要点击次数: 2734 |
| 全文下载次数: 2153 |
| 中文摘要: |
| 目的 探讨电离辐射诱导犬胆管成纤维细胞凋亡及防治胆管损伤后狭窄关系。方法 将103Pd放射性支架,放射性活度为125×104kBq,和普通支架分别植入犬肝外胆管内,取出胆管标本,用DNA凝胶电泳检测成纤维细胞凋亡和透射电镜观察凋亡细胞形态和DNA的变化;并用计算机图像检测系统检测两组胆管腔面积。结果 103Pd放射性支架明显抑制犬胆管组织中增生性瘢痕成纤维细胞生长,并出现典型的细胞凋亡特征,即出现凋亡小体、DNA梯状条带等。而且,103Pd放射性支架犬肝外胆管无明显狭窄,普通支架组犬胆管未出现成纤维细胞凋亡,胆管有明显狭窄。结论 103Pd放射性支架诱导增生性瘢痕成纤维细胞凋亡是防治胆管损伤后由于增生瘢痕导致胆管狭窄的重要机理之一。 |
| 英文摘要: |
| Objective To investigate the role of γ-ray-induced fibroblast apoptosis in the inhibition of biliary duct hypertrophic scar formation in dogs. Methods γ-radiation-induced apoptotic fibroblast cells were analysed by using transmission electron microscopy and DNA from frozen biliary duct tissue was extracted with phenol chloroform.DNA ladder profile after extraction of RNA was observed,and apoptosis cells in paraffineem-bedded biliary duct tissue sections were examined using immuno-histochemical method.Dog biliary duct cross-sections were stained with hematoxylin-erosin,Masson’s trichrome,and Verhoeff-van Giesen stains.Muscle formation area,lumen circumference,and stenosis degree were determined by a computer-assisted image analysis system. Results 103Pd radioactive stent significantly inhibited fibroblast proliferation.The features of fibroblast apoptosis (e.g.apoptic bodies,DNA ladder band) could be seen in the 103Pd radioactive stent group.The fibroblast apoptotic rate was significantly increased in the 103Pd radioactive stent group than in the control group(P<0.01).Comparing with the control group,the 103Pd radioactive stent significantly reduced biliary muscular formation. Conclusion 103Pd radioactive stent could have the effect of inhibiting the proliferation of scar-forming fibroblast,and thus could be used for treatment and (or) prevention of hypertrophic scar formation in biliary duct. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|