| 聂林,张洹,李萍,郭良君,王奕鸣,庄承海.三氧化二砷提高恶性淋巴瘤细胞放射敏感性的观察[J].中华放射医学与防护杂志,2005,25(1):43-45 |
| 三氧化二砷提高恶性淋巴瘤细胞放射敏感性的观察 |
| Radiosensitization of arsenic trioxide on malignant lymphoma cell lines |
| 投稿时间：2004-08-18 |
| DOI： |
| 中文关键词: 三氧化二砷 辐射敏感性 淋巴瘤 Bcl2 凋亡 |
| 英文关键词:Arsenic trioxide Radiosensitivity Lymphoma Bcl-2 Apoptosis |
| 基金项目:广东省医学科学技术研究基金资助项目(B2002069) |
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| 中文摘要: |
| 目的 研究三氧化二砷对恶性淋巴瘤细胞辐射敏感性的影响。方法 采用细胞染色方法观察细胞凋亡的形态,流式细胞仪检测细胞的DNA含量和Bcl2蛋白的表达。结果 0.5～2.0μmolL三氧化二砷和1～4Gyγ射线均能抑制B淋巴瘤细胞株Raji细胞生长,诱导细胞凋亡,流式细胞仪检测Raji细胞亚G1 期细胞明显增多,Bcl2蛋白的表达明显降低,呈现时间剂量依赖效应,联合应用比单独应用抑制作用显著。结论 三氧化二砷能够提高恶性淋巴瘤细胞的放射敏感性。 |
| 英文摘要: |
| Objective To investigate the radiosensitization of arsenic trioxide on malignant lymphoma cell lines. Methods Cell apoptosis situation, cellular DNA contents and Bcl-2 protein expression were determined by cell staining and flow cytometry. Results One to four Gy 60Co γ-irradiation and 0.5- 2.0 μmol/L As2O3 could inhibit Raji cell growth accompanied by the appearance of morphologic characteristics of apoptosis. The number of Sub-G1 cells significantly increased and Bcl-2 protein expression in Raji cells was significantly downregulated as assayed by flow cytometry. The relationship between Bcl-2 protein the concentration of As2O3 and γ-radiation showed both dose- and time-dependent features; so did the number of Sub-G1 cells. The inhibitory effect was much more evident when As2O3 and irradiation were applied combinatively than either one of them was used alone. Conclusion Arsenic trioxide is able to enhance radiation effect on malignant lymphoma cell lines. |
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