| 秦绪军,海春旭,何伟,梁欣,赵康涛,张晓迪,陈宏莉,刘瑞.复合抗氧化剂对局部荷瘤大鼠放疗肾脏损伤的保护作用[J].中华放射医学与防护杂志,2004,24(6):548-550 |
| 复合抗氧化剂对局部荷瘤大鼠放疗肾脏损伤的保护作用 |
| Protective effect of antioxidant compound on renal oxidative injury in locally irradiated rats bearing tumor |
| 投稿时间：2003-04-17 |
| DOI： |
| 中文关键词: 电离辐射 肾脏 肿瘤模型 抗氧化剂 |
| 英文关键词:Irradiation Kidney Tumor model Antioxidant |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨荷瘤大鼠60Coγ射线局部辐射引起的肾脏氧化损伤以及复合抗氧化剂的保护作用。方法 采用Walker256肿瘤细胞株接种大鼠皮下,得到实体瘤,摘除实体瘤分割成小块植入大鼠的右后腿皮下,制成大鼠荷瘤模型,将荷瘤大鼠随机分成3组,分别为肿瘤模型组、单纯放疗组和抗氧化剂保护组,同时选取正常大鼠作为阴性对照组。抗氧化剂保护组每日给予复合抗氧化剂灌胃,分次对单纯放疗组和抗氧化剂保护组的荷瘤大鼠进行60Coγ射线局部照射4次,每次间隔1周,累计剂量为47Gy,最后一次照射后7d,解剖大鼠,取肾脏制成组织匀浆,分别检测丙二醛(MDA)、总超氧化物歧化酶(SOD)和总抗氧化力(TAC)。结果 与阴性组相比单纯放疗组的MDA含量显著升高(P<0.01,P<0.01),抗氧化剂保护组MDA较单纯放疗组显著降低(P<0.05)。单纯放疗组SOD活性和TAC显著低于非照射组(P<0.01,P<0.01),而抗氧化剂保护组SOD和TAC显著高于单纯放疗组(P<0.01,P<0.01)。结论 60Coγ射线局部放疗可以引起肾脏的氧化损伤,高效的复合抗氧化剂可以起到有效的保护作用。 |
| 英文摘要: |
| Objective To observe the oxidative injury and the protective effect of antioxidant compound in kidney induced by local 60Co γirradiation in tumorbearing rats. Methods The tumor cell line Walker256 cells were injected into rats to get solid tumors,which were then removed and cut into small pieces for embedding into the right rear buttocks of rats.As the embedded tumors grew up successfully,the rats were randomly divided into 3 groups:tumor group,irradiation group and protection group.Normal rats served as the controls.The protection group was given antioxidant compound by gavage and other groups were given the same volume of water.The irradiation group and protection group were γirradiated locally with a total dose of 47 Gy in 4 weeks,then all the rats were sacrificed and the kidneys were collected to measure the content of malondialdehyde(MDA),the activities of superoxide dismutase (SOD) and total antioxidant capacity(TAC). Results The content of MDA in the irradiation group was much higher than that in other groups(P<0.05).The activities of TSOD and TAC in the irradiation group were much lower than those in other groups.(P<0.01,P<0.01). Conclusion Local irrdiation can induce oxidative injury to the kidney of the rats,and the antioxidant compound sows good protective effect. |
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