| 封江彬,陆雪,陈德清,陈晓穗,刘青杰.巢式PCR分析电离辐射诱导人外周血线粒体DNA4977bp缺失[J].中华放射医学与防护杂志,2004,24(6):533-536 |
| 巢式PCR分析电离辐射诱导人外周血线粒体DNA4977bp缺失 |
| Detection the human mitochondrial DNA 4977 bp deletion induced by 60Co γ-rays in vitro by nest-PCR |
| 投稿时间：2004-05-09 |
| DOI： |
| 中文关键词: 线粒体DNA4977bp缺失 60Coγ射线照射 巢式PCR |
| 英文关键词:Mitochondrial DNA 4977 bp deletion 60Co γ-rays Nest-PCR |
| 基金项目:人事部留学回国人员择优资助项目(2002年 优秀类) |
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| 中文摘要: |
| 目的 探索对不同剂量电离辐射诱导的人外周血线粒体DNA4977bp缺失进行分析的方法。方法 采用3对引物对γ射线诱导的人外周血线粒体DNA缺失进行PCR扩增,建立对人线粒体DNA4977bp缺失进行分析的巢式PCR方法,扩增线粒体DNA缺失区片段,纯化PCR产物进行测序。采集5例正常人外周血进行0～5Gy60Coγ射线的外照射,利用巢式PCR技术,分析60Coγ射线照射后外周血有核细胞中线粒体DNA4977bp缺失的发生情况。结果 用建立的巢式PCR方法可在受到照射的外周血样品中检测到mtDNA4977bp缺失。所有外周血样本在照射前均无线粒体DNA4977bp缺失,1～5Gy60Coγ线照射后均诱导出此缺失。结论 本研究所建立的巢式PCR方法可用来分析电离辐射诱导的人外周血淋巴细胞线粒体DNA4977bp缺失。 |
| 英文摘要: |
| Objective To establish a method for detecting the mitochondrial DNA 4977 bp deletion (mtDNA 4977) induced by different doses of ionizing radiation. Methods A nest-PCR method was established with 3 primer pairs for detecting the human peripheral mtDNA 4977.The final PCR products were sequenced after purified and the sequence was BLASTed with the standard genome information of human mitochondrion. The mtDNA 4977 level induced by 05 Gy 60Co γ-rays of 5 healthy individuals was analyzed with the established nest-PCR. Results The mtDNA 4977 could be detected by the established nest-PCR method. The mtDNA 4977 was observed on samples after exposed to 15 Gy 60Co γ-rays,but it was not observed before (0 Gy) exposure. Conclusion The nest-PCR method established in this study could be used to detect the mtDNA 4977 induced by ionizing radiation. |
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