| 范才,张建军,李秀丽,徐莉,陈卫辉,鞠桂芝.电离辐射对小鼠组织金属硫蛋白-1mRNA表达的影响[J].中华放射医学与防护杂志,2004,24(6):525-526 |
| 电离辐射对小鼠组织金属硫蛋白-1mRNA表达的影响 |
| Effect of ionizing radiation on MT-1 mRNA expression of mouse tissues |
| 投稿时间：2004-04-26 |
| DOI： |
| 中文关键词: 金属硫蛋白 电离辐射 mRNA |
| 英文关键词:Metallothionein Ionizing radiation mRNA |
| 基金项目:国家自然科学基金资助项目(39170275) |
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| 中文摘要: |
| 目的 探讨X射线全身照射对小鼠肝、脑、胸腺及脾脏金属硫蛋白(MT)1mRNA表达的影响及其剂量效应关系。方法 采用Northern杂交方法检测了X射线全身照射后小鼠肝、脑、胸腺及脾脏MT1mRNA水平变化。结果 4GyX射线照射后肝脏及胸腺MT1mRNA水平逐渐升高,照射后4h达峰值,分别为假射组的1.82及1.72倍,24h基本恢复至正常水平;0.5～6Gy照射后4h,肝脏及胸腺MT1mRNA水平均呈剂量依赖性增加,6Gy照射后MT1mRNA水平分别为假照组的2.13和1.62倍。但X射线全身照射后小鼠脑及脾脏MT1mRNA水平未见明显变化。结论 X射线全身照射可提高小鼠肝脏和胸腺MT1mRNA水平,但对小鼠脑及脾脏MT1mRNA表达无影响。 |
| 英文摘要: |
| Objective To investigate the effect of whole body X-irradiation (WBI) on metallothionein-1 (MT-1) gene transcription level in mouse tissues and its dose-effect relationship. Methods MT-1 mRNA levels in mouse liver,brain,thymus and spleen were measured using Northern blot assay. Results Time-course experiment showed that MT-1 mRNA levels in mouse liver and thymus gradually increased after WBI,reached a peak at 4 h after WBI and recovered normal level at 24 h. MT-1 mRNA levels in mouse liver and thymus increased with increase of irradiation dose at 4 h after 0.5～6 Gy X-ray WBI. But there was no obvious change of MT-1 mRNA levels in mouse brain and spleen. Conclusion MT-1 mRNA levels in mouse liver and thymus significantly increase after WBI and showed a dose-dependent trend. But there is no obvious change in MT-1 mRNA levels in mouse brain and spleen. These Results indicate that increase of MT-1 mRNA in mouse tissues after WBI is tissue-specific,and MT protein synthesis is regulated through MT-1 gene transcriptional elevation. |
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