| 赵剑,韩云,朱应葆.异染色质蛋白HP1α参与辐射损伤修复的研究[J].中华放射医学与防护杂志,2004,24(6):493-495 |
| 异染色质蛋白HP1α参与辐射损伤修复的研究 |
| Heterochromatic protein HP1α is involved in repair of radiation damage |
| 投稿时间：2004-07-15 |
| DOI： |
| 中文关键词: 辐射损伤修复 异染色质 HP1 HR24L |
| 英文关键词:Radiation damage repair Heterochromatin HP1α HR24L |
| 基金项目:国家自然科学基金资助项目(30070232) |
|
| 摘要点击次数: 2189 |
| 全文下载次数: 1902 |
| 中文摘要: |
| 目的 研究HP1α对辐射损伤修复的影响。方法 用反义技术单独抑制HP1α和联合抑制HR24L表达,观察细胞对辐射敏感性的影响,用免疫共沉淀法观察HP1α是否参与修复蛋白复合物的形成。结果 单独抑制HP1α,细胞对辐射敏感性与正常细胞相比差异无显著性,联合抑制HP1α和HR24L时,细胞对辐射的敏感性比单独抑制HR24L时显著增加;20Gyγ射线照射后8h,可检测出HR24L与HP1α复合物的存在。结论 HP1α与修复蛋白相互作用,参与DNA损伤修复,从而影响细胞对辐射的敏感性。 |
| 英文摘要: |
| Objective To investigate the role of HP1α in DNA repair induced by ionizing radiation. Methods Inhibition of HP1α and HR24L by antisense RNA,respectively and combinatively was used to observe the sensitivity of cells to ionizing radiation,and coimmuoprecipitation was performed to find HP1αcontaining HR24L complex. Results Inhibition of HP1α expression had no obvious effect on radiation sensitivity of cells.Significantly,double inhibition of both HP1α and HR24L led to increased sensitivity of cells to ionizing radiation than sigle inbihition of HR24L.In addition,HP1α was found to coprecipitated with HR24L after DNA damage induced by ionizing radiation with 20 Gy γ-rays. Conclusion HP1α participates in the formation of HR24L complex that is involved in DNA damage repair,thus contributing to the radiation sensiivity of cells. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |