| 冯惠茹,田嘉禾,丁为民,张锦明,陈英茂.β和γ射线照射肿瘤细胞的生物效应比较[J].中华放射医学与防护杂志,2004,24(5):416-418 |
| β和γ射线照射肿瘤细胞的生物效应比较 |
| A comparative study of biological effects on tumor cells between low-dose-rate β-irradiation and high-dose-rate γ-irradiation |
| 投稿时间：2003-10-18 |
| DOI： |
| 中文关键词: HeLa细胞系 低剂量率辐射 高剂量率辐射 肿瘤杀伤效应 |
| 英文关键词:HeLa cell line Low-dose-rate irradiation High-dose-rate irradiation Cell-killing effect |
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| 中文摘要: |
| 目的 比较低剂量率β射线和高剂量率γ射线照射诱发肿瘤细胞生物效应特点。方法 采用32Pβ射线和60Coγ射线照射人宫颈癌HeLa细胞系,用台盼蓝排除法和Xgal衰老细胞染色法比较两种照射肿瘤细胞死亡方式的差异。结果 32Pβ射线(0.375cGymin)和60Coγ射线(206cGymin)照射HeLa细胞后72h的结果表明,低剂量率β射线抑制细胞增殖为渐进性,使多数的细胞在一个或几个细胞倍增周期后死亡,以增殖性死亡为主;高剂量率γ射线对细胞的抑制作用直接、迅速,细胞坏死比例高,增殖性死亡(衰老)比例低于持续低剂量照射方式。结论 不同的辐射方式对细胞的杀伤方式不同,了解其放射生物学机理有助于临床治疗方案的选择。 |
| 英文摘要: |
| Objective To elucidate the radiobiological mechanism underlying cancer treatment with low-dose-rate β-irradiation of 32P,by comparing the cell-killing pattern with high-dose-rate γ-irradiation of 60Co. Methods HeLa cells were exposed to low-dose-rate β-irradiation of 32P or high-dose-rate γ-irradiation of 60Co. Comparisons were made between these two types of radiations,focusing on the cell-killing patterns evaluated by radiation-induced reproductive cell death(senescence)and necrotic cell death through X-gal senescence staining and typan blue exclusion method respectively. Results The results showed that there were different cell-killing patterns in HeLa cells between 32P radiation at dose-rate of 0.375 cGy/min and that of 60Co at dose-rate of 206 cGy/min at 72 h post irradiation. In exposure to 32P,more HeLa cells were lost due to reproductive cell death than to 60Co. X-gal staining showed that senescent cell ratio in HeLa cells after exposure of 32P was higher than after that of 60Cowhich caused more necrotic cell death. Conclusions More reproductive and less necrotic cell death contribute to total cell loss in HeLa cells by radiation of 32P than by 60Co.Understanding the underlying mechanism of radiobiology in different radiations is helpful in radiotherapy of malignant tumor by choosing a more reasonable and effective procedure of treatment. |
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