| 董丽华,刘光伟,朴春南,吕文天,赵红光,贾晓晶,赵勇,龚守良.信号因子对辐射诱导胸腺淋巴细胞凋亡的影响[J].中华放射医学与防护杂志,2004,24(5):399-402 |
| 信号因子对辐射诱导胸腺淋巴细胞凋亡的影响 |
| Effect of signal factors on lymphocyte apoptosis in mouse thymus induced by ionizing radiation in vitro |
| 投稿时间：2004-02-04 |
| DOI： |
| 中文关键词: 信号因子 电离辐射 淋巴细胞 凋亡 |
| 英文关键词:Signal factors Ionizing radiation Lymphocytes Apoptosis |
| 基金项目:国家自然科学基金资助项目(39170275) |
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| 摘要点击次数: 2694 |
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| 中文摘要: |
| 目的 观察信号因子皮质酮(CS)、cAMP、cGMP、Ca2+和蛋白激酶C(PKC)对体外4GyX射线照射诱导小鼠胸腺淋巴细胞凋亡的影响。方法 应用荧光发光分光光度仪检测胸腺淋巴细胞DNA裂解率。结果 2～8Gy照射后4～8h,胸腺淋巴细胞DNA裂解率均明显高于对照组(P<0.01)。与对照组比较,加入0.01μmolLCS、50ng/mlcAMP、0.05～04μgmlCa2+结合载体离子霉素(Iono)和0.05～0.4ng/mlPKC激活剂豆蔻酰佛波醇乙酯(PMA)均明显增加淋巴细胞DNA裂解率,P<0.05或<0.01,而加入50ng/mlcGMP与对照组比较,无明显作用。对0.01μmolLCS、50ng/mlcAMP、0.2和0.4μgmlIono及0.2和0.4ng/mlPMA分别加4Gy照射,其DNA裂解率均明显高于单纯4Gy照射组,P<0.05或<0.01,而50ng/mlcGMP加4Gy照射与单纯4Gy照射组比较无明显差异。结论 在一定剂量范围内,信号因子CS、cAMP、Ca2+和PKC对大剂量X射线照射诱导小鼠胸腺淋巴细胞凋亡具有促进作用。 |
| 英文摘要: |
| Objective To observe the effects of corticosterone (CS),cAMP,cGMP,Ca2+ and protein kinas C(PKC) signal factors on lymphocyte apoptosis in mouse thymus induced by ionizing radiation of 4 Gy X-rays in vitro. Method The DNA lytic rate of lymphocytes in thymus was measured with fluorospectrophotometry. Results The DNA lytic rates of lymphocytes in thymus 4-8 h after irradiation with 2-8 Gy were all significantly higher than those of the control.As compared with the control,the DNA lytic rates of lymphocytes all increased significantly in 0.01 μmol/L CS(P<0.01),50 ng/ml cAMP (P<0.01),0.05-0.4 μg/ml ionomycin (Iono,(P<0.05) or P<0.01) and 0.05-0.4 ng/ml phorbol myristate acetate (PMA,P<0.05 or P<0.01) groups,respectively,while the rate in 50 ng/ml cGMP did not increase significantly.The DNA lytic rates of lymphocytes were significantly higher in 0.01 μmol/L CS(P<0.01),50 ng/ml cAMP (P<0.01),0.2 and 0.4 μg/ml Iono (P<0.05) and 0.2 and 0.4 ng/ml PMA (P<0.05) plus 4 Gy-irradiation groups respectively than that in single 4 Gy-irradiation group,while that in 50 ng/ml cGMP plus 4 Gy-irradiation group was not.As both Iono and PMA were combined to act to the lymphocytes,the DNA lytic rate of only in 0.4 μg/ml Iono and 0.4 ng/ml PMA group was significantly higher than that in the control (P<0.01);the rate in 0.4 μg/ml Iono and 0.4 ng/ml PMA plus 4 Gy-irradiation group was significantly higher than that in the single 4 Gy-irradiation group (P<0.05),but was not significantly higher than that in the 0.4 μg/ml Iono plus 4 Gy-irradiation group or 0.4 ng/ml PMA plus 4 Gy-irradiation group. Conclusion There are promoting effects of CS,cAMP,Ca2+ and PKC signal factors in certain dose ranges on lymphocyte apoptosis in mouse thymus induced by ionizing radiation with larger dose of X-rays. |
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