| 刘振芳,孙汉英,刘文励,李春蕊,谢大兴,吴宁.川芎嗪促进急性放射损伤小鼠骨髓造血修复作用的研究[J].中华放射医学与防护杂志,2004,24(5):396-398 |
| 川芎嗪促进急性放射损伤小鼠骨髓造血修复作用的研究 |
| Study on the effects of ligustrazine on recovery of hematopoiesis in acute irradiation injured mice |
| 投稿时间：2003-10-07 |
| DOI： |
| 中文关键词: 川芎嗪 放射损伤 血管内皮生长因子 |
| 英文关键词:Ligustrazine Radiation injury VEGF |
| 基金项目:国家自然科学基金资助项目(39870926) |
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| 中文摘要: |
| 目的 探讨川芎嗪促进急性放射损伤小鼠骨髓造血修复可能的机理。方法 取56只昆明小鼠,随机分为3组:正常组、对照组和川芎嗪组。对照组和川芎嗪组进行60Coγ射线一次性全身均匀照射,总吸收剂量为6.0Gy,吸收剂量率为0.56Gymin,照射后分别胃饲相同剂量(2mg只,2次d)的生理盐水和川芎嗪,分别于照射后第3、7、14天计数骨髓单个核细胞(BMNC),检测骨髓组织中造血组织面积和血管内皮生长因子(VEGF)及骨髓单个核细胞PECAM-1的表达水平。结果 川芎嗪组第3、7、14天BMNC计数及骨髓造血组织面积均显著高于对照组(P<0.01);川芎嗪组VEGF、PECAM-1的表达水平在第3、7天明显高于对照组(P<0.01),而第14天其表达降低并明显低于对照组(P<0.05)。结论 川芎嗪可能通过增强骨髓中黏附分子PECAM-1和VEGF的表达,以促进急性放射损伤后造血细胞和造血微环境的修复。 |
| 英文摘要: |
| Objective To explore the effect and possible mechanism of ligustrazine on the recovery of hematopoiesis in acute irradiation-injured mice. Methods The mice were divided into 3 groups:normal group,irradiation-injured group and ligustrazine group.After irradiation by 6.0 Gy 60Coγ-rays,each mouse was given 2 mg ligustrazine orally twice a day for 13 days in the ligustrazine group,and was given equal quantity of saline in the irradiation-injured group.On days 3,7,14 after irradiation,bone marrow mononuclear cells (BMNC) were counted,the percentage of hematopoietic tissue and the expression levels of VEGF and PECAM-1 in bone marrow were evaluated. Results On days 3,7,14 after irradiation,in the ligustrazine group the BMNC counts and the percentage of hematopoietic tissue were higher than those in the irradiation injured group (P<0.01).The PECAM-1 and VEGF expression levels in the ligustrazine group were higher than in the irradiation-injured group on days 3,7 (P<0.01) and lower than in the latter on day 14 (P<0.05). Conclusion Ligustrazine can enhance VEGF and PECAM-1 expression in bone marrow in acute irradiation-injured mice,which may be one of the mechanisms of ligustrazine in accelerating the recovery of hematopoiesis in mouse bone marrow. |
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