| 姚莉,梁晓俐,王德文,眭翔,王春平,王水明,张莉,高亚兵,彭瑞云.高场强电磁脉冲辐射对小鼠肝细胞DNA含量和倍体影响的定量学研究[J].中华放射医学与防护杂志,2004,24(4):386-388 |
| 高场强电磁脉冲辐射对小鼠肝细胞DNA含量和倍体影响的定量学研究 |
| Quantitative study of the effects of high power EMP on hepatocellular DNA content and ploidy in mice |
| 投稿时间：2003-10-16 |
| DOI： |
| 中文关键词: 电磁辐射 电磁脉冲 肝 DNA含量 DNA倍体分型 图像细胞光度计 |
| 英文关键词:Electromagnetic irradiation Electromagnetic pulse (EMP) Liver DNA content DNA diploid cell type Image cytophotometry(ICM) |
| 基金项目:全军“十五”军队科研基金资助项目(01MB143,01L023) |
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| 中文摘要: |
| 目的 动态观察高场强电磁脉冲辐射(EMP)对小鼠肝细胞核DNA含量及倍体的影响,探讨电磁辐射的生物学效应。方法 应用高场强EMP发射源对二级昆明小鼠进行全身辐射,场强分别选用8×103、2×104和6×104Vm,发射源相关技术参数:脉冲上升时间20ns,脉宽30μs,2min内发射5个单脉冲;采用Feulgen染色动态观察小鼠肝细胞核内DNA含量的变化,观察时间1年,共设10个时相点(n=6),并用德国IBSA显微数字图像分析系统作DNA含量定量分析和倍体分型。结果 小鼠经高场强EMP辐射后3个月内,肝细胞核DNA含量与正常对照组差异无显著性,以二倍体细胞(2C)为主,四、六倍体(4C,6C)较少,八倍体(8C)偶见;辐射后6个月,8×103Vm辐射组比对照组DNA含量高(P<0.05),2C数量减少,4C和6C增加;至辐射后9个月和12个月,各辐射组肝细胞核DNA含量比其他各时相点辐射组及同一时相点对照组显著增高(P<0.01),并且以4C为主,6C和8C增加,而2C明显减少。结论 高场强EMP对小鼠肝DNA含量及倍体有影响,且表现为远后效应,推测电磁辐射对肝的生物学效应中肝细胞核酸可能是一个重要的靶点,这将为进一步研究其损伤效应及其作用机制提供实验性依据。此外，本研究结果提示要注重电磁辐射远后效应的研究，以确保研究结果的完整性与可靠性，为其损伤防治提供新思路。 |
| 英文摘要: |
| Objective To observe the changes of high power electromagnetic pulse(EMP) on DNA content and DNA ploidy of mice hepatocytes and explore the biological effects of electromagnetic radiation on liver. Methods Kunming mice were exposed to whole body irradiation by a HEMP generator at electric field intensity of 8×10 3 V/m, 2×10 4 V/m, 6×10 4 V/m, respectively. The main relative technological parameters were: the pulse increase time was 20 nsec, pulse width 30 μs; and irradiation frequency 5 times in 2 min. The changes of hepatocellular nuclear DNA content in mice were analyzed for 365 days including 10 time phases (n =6)by using Feulgen staining in liver tissue sections. DNA content quantitation and DNA ploidy were measured by an IBAS microscopical digital image analysis system from German. Results Within 90 days after HEMP irradiation, the hepatocellular DNA content of mice were similar to that of the controls and the 2 C were dominant. On day 180 after irradiation, the higher DNA content (P <0.05) and more 4 C, 6 C than controls in 8×10 3 V/m group were found. On days 270 and 365, in all irradiation groups, the highest DNA content (P <0.01) and most 4 C were presented than the other time phases. Conclusion The results suggest that the DNA content and ploidy of hepatocyte in mice can be altered by HEMP and thus may be the long-term effects. It is proposed that the hepatocellular nucleic acid may be a key target for the biological effects of electromagnetic fields. The data provide basic information for studying the effects and mechanisms of EMP. Moreover, the investigation demonstrated that long-term observation must be emphasized in order to keep the integrity and reliability of results for gainning further insight into prevention and therapy for electromagnetic irradiation injury. |
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