| 李俊明,周敬群,胡斌,等.小剂量放疗对弹性蛋白及弹性蛋白酶mRNA表达的影响[J].中华放射医学与防护杂志,2004,24(4):342-344.LI Jun-ming,ZHOU Jing-qun,HU Bin,et al.Effects of brachytherapy on gene expressions of elastin and elastase[J].Chin J Radiol Med Prot,2004,24(4):342-344 |
| 小剂量放疗对弹性蛋白及弹性蛋白酶mRNA表达的影响 |
| Effects of brachytherapy on gene expressions of elastin and elastase |
| 投稿时间：2003-10-30 |
| DOI： |
| 中文关键词: 近距离放射治疗 弹性蛋白 弹性蛋白酶 |
| 英文关键词:Brachytherapy Elastin Elastase |
| 基金项目:湖北省教委重点基金资助项目(2000B14018) |
| 作者 | 单位 | | 李俊明 | Department of Cardiology, Renhe Hospital, Three Gorges University, Yichang 443001, China | | 周敬群 | Department of Cardiology, Renhe Hospital, Three Gorges University, Yichang 443001, China | | 胡斌 | Department of Cardiology, Renhe Hospital, Three Gorges University, Yichang 443001, China | | 李书国 | Department of Cardiology, Renhe Hospital, Three Gorges University, Yichang 443001, China |
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| 中文摘要: |
| 目的 探讨小剂量放射治疗对血管弹性蛋白及弹性蛋白酶mRNA基因表达的影响。方法 以0、7、14和28Gy60Coγ射线照射培养的大鼠主动脉平滑肌细胞,用逆转录竞争性聚合酶链反应观察照射培养的大鼠主动脉平滑肌细胞弹性蛋白和弹性蛋白酶mRNA水平。结果 7Gy照射对血管平滑肌细胞弹性蛋白和弹性蛋白酶mRNA的表达无影响,14和28Gy照射后细胞弹性蛋白mRNA水平较对照组增加了80.7%及10.23%(P<0.05),细胞弹性蛋白酶mRNA水平较对照组减少了25.3%及50.1%(P<0.05)。结论 提示14和28Gy60Coγ射线照射通过抑制弹性蛋白酶及增加弹性蛋白合成而影响血管细胞外基质的代谢。 |
| 英文摘要: |
| Objective To study the effects of brachytherapy on the gene expessions of elastin and elastase in cultured rat vascular smooth muscle cells (VSMCs). Methods Rat VSMCs cultured in DMEM containing 10% FBS were irradiated by60 Co γ-rays at 0, 7, 14, 28 Gy respectively. Then mRNA levels of elastin and elastase were determined by reverse transcription competitive PCR(RT-PCR). Results Brachytherapy inhibited the expressions of elastase. Elastase mRNA decreased from 82.52±6.50 to 64.25±3.81 and 42.17±3.16 in VSMC irradiated with 14, 25 Gy, respectively(P <0.05). The elastin mRNA level increased from 95.17±6.41 to 72.05±5.82 and 49.77±5.62 in VSMC irradiated with 14, 25 Gy, respectively(P <0.05). Conclusion Brachytheapy inhabits the expressions of elastase and increased elastin in VSMC cells. |
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