| 赵永成,田素青,王继先,等.荧光原位杂交微核技术在辐射生物剂量学的应用研究[J].中华放射医学与防护杂志,2004,24(4):333-335.ZHAO Yong-cheng,TIAN Su-qing,WANG Ji-xian,et al.Study on the applications of FISH micronucleus technique in radiation biological dosimetry[J].Chin J Radiol Med Prot,2004,24(4):333-335 |
| 荧光原位杂交微核技术在辐射生物剂量学的应用研究 |
| Study on the applications of FISH micronucleus technique in radiation biological dosimetry |
| 投稿时间：2003-04-08 |
| DOI： |
| 中文关键词: 电离辐射 荧光原位杂交 微核分析 X射线工作者 生物剂量计 |
| 英文关键词:Ionization radiation Fluorescence in situ hybridization MN assay X-ray workers Biological dosimeter |
| 基金项目:日本体质研究会基金资助项目;国家自然科学基金资助项目(30070644) |
| 作者 | 单位 | | 赵永成 | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China | | 田素青 | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China | | 王继先 | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China | | 范亚光 | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China |
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| 摘要点击次数: 3111 |
| 全文下载次数: 1995 |
| 中文摘要: |
| 目的 探讨荧光原位杂交(FISH)微核作为生物剂量计及评价职业受照射人群辐射效应的可行性。方法 用137Csγ射线不同剂量(0.1～2.5Gy)照射离体人外周血细胞,用泛着丝粒探针的FISH技术进行微核分析,拟合剂量效应方程;检测医用X射线工作者的无着丝粒微核率并估算其生物剂量。结果 离体照射条件下微核率随吸收剂量增加而增加,以无着丝粒微核增加为主,着丝粒微核仅有轻度增加。微核率与剂量之间的拟合曲线方程,CB微核法:Y=0.005+0.036D+0.011D2,R2=0.99;FISH微核法:Y=0.001+0.035D+0.007D2,R2=0.99。结论 FISH微核法优于CB微核法。用FISH微核法对医用X射线工作者的检测结果显示无着丝粒微核率可以用来估算生物剂量。 |
| 英文摘要: |
| Objective To investigate the feasibility of FISH micronucleus technique as a bio-dosimeter and evaluation the radiation effect of occupational population who exposed to radiation. Methods human peripheral blood samples were irradiated in vitro with 137 Cs γ rays with the doses ranging from 0.1 to 2.5 Gy, then CBMN was analyzed using FISH with Pancentromere DNA probe, and dose-response equation was estimated. And by detecting of the centromere-negative micronuclei rate of occupational exposure of the medical X-ray diagnostic workers in our country, the biological individual dose was estimated. Results Irradiatied in vitro, the micronucleus frequencies increased with the increase of radiation dose, and centromere-negative micronuclei accounted for the main increase of micronucleus and centromere-positive micronuclei only slightly increased. the dose-response relationships for CBMN assay and FISH assay were: YCBMN = 0.0048 + 0.0362D + 0.0109D2; R 2= 0.99; YFISH = 0.0007+ 0.0353D +0.0070D 2, R2= 0.99, respectively. Conclusions FISH micronucleus assay decreased the individual variation of spontaneous micronucleus, enhanced the radiation specificity of MN assay and was better than CBMN. The result shows that centromere-negative micronuclei rate can be used to estimate to biological dose. |
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