| 刘建香,薛丽香,苏旭.电离辐射调控脂质体介导的p16基因在HeLa细胞中的表达[J].中华放射医学与防护杂志,2004,24(4):327-330.LIU Jian- xiang,XUE Li- xiang,SU Xu.Ionizing radiation regulated expression of Lipofectamin-mediated p16 gene in HeLa cells[J].Chin J Radiol Med Prot,2004,24(4):327-330 |
| 电离辐射调控脂质体介导的p16基因在HeLa细胞中的表达 |
| Ionizing radiation regulated expression of Lipofectamin-mediated p16 gene in HeLa cells |
| 投稿时间：2004-01-18 |
| DOI： |
| 中文关键词: Egr-1基因 p16基因 p16mRNA 细胞周期 细胞生长曲线 |
| 英文关键词:Egr-1 gene p16 gene p16 mRNA Cell cycle Cell growth curve |
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| 中文摘要: |
| 目的 探讨不同剂量γ射线照射后诱导脂质体介导的p16基因在HeLa细胞中的表达及抗癌作用。方法 用脂质体Lipofectamin介导重组质粒Egr-p16转染人HeLa细胞,采用RTPCR的方法检测了60Coγ射线照射转染后的人HeLa细胞剂量效应和时程变化,用细胞计数检测细胞增殖的变化,用流式细胞术检测细胞周期的变化。结果 研究证实0.5～8Gy照射后p16的转录水平高于对照,在2～4Gy照射后达到峰值,2Gy照射后2～24h高于对照组,在照射后4h达到峰值,然后逐渐下降。细胞生长曲线显示体外稳定转染联合60Coγ射线照射对HeLa细胞的增殖具有明显的抑制作用。细胞周期变化显示转染后的HeLa细胞经过照射后G0G1期比例呈现剂量依赖性的下降,而S期则出现剂量依赖性的增加,出现明显的S期阻滞,G2/M期在2Gy出现明显的阻滞,在5和10Gy时逐渐下降,但是仍然高于对照组。结论 60Coγ射线可诱导转染的HeLa细胞p16转录水平的增强,同时在细胞增殖上出现明显的变化。 |
| 英文摘要: |
| Objective To investigate the expression of Egr-p16 and its role in tumor cell inhibition in HeLa cells. Methods HeLa cells were transfected with p16 by recombined plasmids with Lipofectamin induced by γ-ray irradiation with different doses. RT-PCR was used to detect p16 mRNA level in the dose-effect and time-course aspects. Cell proliferation was detected by cell count and cell cycle changes were detected by FCM. Results The results showed that p16 mRNA level was higher than the control after 0.5-8.0 Gy irradiation. There were peaks after 2-4 Gy irradiation, and p16 mRNA level increased at 2-4 h after 2 Gy irradiation. There was a significant increase at 4 h. After the stably transfected HeLa cells were irradiated, the transfer of Egr-p16 combined with γ-ray irradiation could inhibit the growth of HeLa cells. The percentage of G0/G1 showed a dose-dependant decrease, but the percentage of S showed a dose-dependant increase. The percentage of G2/M showed a markedly arrest after 2 Gy irradiation, and gradually reduced after 5 and 10 Gy irradiation, but it was still higher than the control group. Conclusion γ-rays can induce the recombined plasmid to express at mRNA level in HeLa cells and there was a significant change in cell proliferation. |
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