| 管晓翔,陈龙邦,于正洪,等.60Co γ射线对肝癌细胞株HepG2增殖和细胞周期的影响[J].中华放射医学与防护杂志,2004,24(4):311-313.GUAN Xiao-xiang,CHEN Long-bang,YU Zheng-hong,et al.Cell cycle alteration and cell growth inhibition induced by 60Co γ-irradiation in HepG2 cell line[J].Chin J Radiol Med Prot,2004,24(4):311-313 |
| 60Co γ射线对肝癌细胞株HepG2增殖和细胞周期的影响 |
| Cell cycle alteration and cell growth inhibition induced by 60Co γ-irradiation in HepG2 cell line |
| 投稿时间：2004-01-26 |
| DOI： |
| 中文关键词: 肿瘤细胞HepG2 60Co γ射线 细胞周期 p27kip1 |
| 英文关键词:HepG2 60Co γ-ray Cell cycle p27kip1 |
| 基金项目:中国博士后科学基金资助项目(2003034383) |
| 作者 | 单位 | E-mail | | 管晓翔 | Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China | longbangchen@yeah.com | | 陈龙邦 | Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China | | | 于正洪 | Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China | | | 鹿红 | Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China | | | 王靖华 | Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China | |
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| 摘要点击次数: 2933 |
| 全文下载次数: 1876 |
| 中文摘要: |
| 目的 观察60Coγ射线对肝癌细胞株HepG2增殖、凋亡和细胞周期的影响,并探讨γ射线对细胞周期改变与p27kip1蛋白表达和分布之间的关系。方法 以不同剂量的γ射线照射培养的HepG2细胞,观察细胞形态和生长曲线变化,同时应用流式细胞计数仪观察细胞周期和凋亡的改变,并应用免疫荧光方法观察p27kip1蛋白的表达和分布改变。结果 60Coγ射线照射细胞引起HepG2细胞株剂量依赖性的生长抑制和凋亡增加。60Coγ射线可引起细胞G2M期阻滞,同时p27kip1表达明显抑制,并主要分布于细胞浆。结论 60Coγ射线所导致的肝癌细胞株HepG2细胞的G2M阻滞可能与细胞内p27kip1的表达抑制和细胞浆的分布比例增加有关。 |
| 英文摘要: |
| Objective To study the cell cycle alterations in cells of human liver cancer cell line HepG2 in vitro after 60Co γ-irradiation; in order to investigate further the role of p27 kip1 protein on cell cycle and proliferation. Methods The effect on proliferation of HepG2 cell line by irradiation with 1.0-6.0 Gy γ-rays was evaluated with MTT assay. The cell cycle profile and apoptosis were analyzed by FACS, and the expression and subcellular localization of p27kip1 in the cells were detected by laser confocal microscopy. Results 60 Co γ-irradiation inhibited the growth of HepG2 cell line in a dose-dependent manner. The HepG2 cell line cells were arrested in G2/M 48 hours after 3.0 Gy γ-ray exposure. The expression of p27 kip1was down-regulated as compared to HepG2 cell line cells without γ- radiation exposure, Furthermore the p27kip1 was redistributed to cytoplasm after γ-irradiation. Conclusion The low expression and cytoplasmic localization of p27 kip1 might be due to the G2/M phase arrest induced by gamma- irradiation of HepG2 cell line cells. |
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