| 张珊文,肖绍文,杨敬贤.腺病毒介导p53基因转染增加人胃癌细胞的放射敏感性[J].中华放射医学与防护杂志,2004,24(4):308-311 |
| 腺病毒介导p53基因转染增加人胃癌细胞的放射敏感性 |
| Adenovirus-mediated p53 gene transfer increases radiosensitivity of human gastric carcinoma cells |
| 投稿时间：2003-08-15 |
| DOI： |
| 中文关键词: 胃癌 放疗 腺病毒介导p53基因 |
| 英文关键词:Gastric carcinoma Radiotherapy Adenovirus-mediated p53 gene |
| 基金项目:国家自然科学基金资助项目(39670234) |
|
| 摘要点击次数: 2578 |
| 全文下载次数: 1577 |
| 中文摘要: |
| 目的 评价腺病毒介导p53基因(Adp53)转染对人胃癌细胞的凋亡效应和放射增敏作用。方法 以重组腺病毒介导p53 基因感染4种不同p53 状况的人胃癌细胞,用免疫组织化学法和Westernblot法检测P53 蛋白在胃癌细胞中的表达;用细胞集落形成法检测细胞存活率;用TUNEL法检测细胞凋亡。胃癌细胞感染Adp53后照射4Gy,用流式细胞仪检测细胞周期分布和凋亡;胃癌细胞种植肿瘤内注射Adp53后照射6Gy,以肿瘤相对体积增长曲线观察肿瘤抑制情况。结果 1:100效靶比(MOI)Adp53产生细胞高转染率,以及p53 基因在4种胃癌细胞中均高表达,并产生G2M期阻滞、凋亡增加和细胞增殖抑制。如果以凋亡评价放射效应,Adp53转染对4Gy照射4种细胞的凋亡率比值为:W细胞30,M细胞36,neo细胞22,823细胞2.5。体内实验结果显示,Adp53对W细胞肿瘤6Gy照射的抑瘤率比值为1.41,而对M细胞肿瘤为1.91。结论 腺病毒介导p53 基因转染产生细胞凋亡并提高人胃癌细胞的放射敏感性,这种作用不依赖于细胞内在的p53 状况。 |
| 英文摘要: |
| Objective To evaluate the effect of adenovirus-mediated p53 gene (Adp53) on apoptosis and radiosensitivity of human gastric carcinoma cell lines. Methods Recombinant adenovirus carrying wild-type p53 gene was transferred into four human gastric carcinoma cell lines with different p53 genetic status. P53 protein expression was detected by immunohistochemistry and Western blot assay. Cell survival was assessed using a clonogenic assay. TUNEL assay was used in determination of apoptosis. The four human gastric carcinoma cell line infected with Adp53 were irradiated with 4 Gy, and cell cycle distribution and apoptotic rate were assayed by flow cytometry. Nude mice xenograft models of W and M cell were intratumorally injected with Adp53 and 48 h later were irradiated with 6 Gy. Relative volume in growth curve of tumor was used to observe tumor regression. Results G2/M arrest, apoptosis and inhibition of tumor cell proliferation were induced by infection with Adp53 at 100 MOI, which caused high transfer rate of wild-type p53 and strong expression of P53 protein in the four human gastric carcinoma cell line cells. When evaluating radio-biologic efficacy by apoptotic rate, the apoptotic enhencement ratio of Adp53 at 4 Gy was 3.0 for W cell, 3.6 for M cell, 2.2 for neo cell and 2.5 for 823 cell respectively, in vitro. The antitumor enhencement ratio of Adp53 at 6 Gy was 1.41 for W cell-implanted tumor and 1.91 for M cell-implanted tumor in vivo. Conclusion This study demonstrated that Adp53 transfer increased cellular apoptosis and radiosensitivity of human gastric carcinoma. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|