| 雷苏文,涂序珉,王菊芳,罗光辉,李文建.辐射诱发肿瘤细胞旁效应的免疫学实验研究[J].中华放射医学与防护杂志,2004,24(4):300-301 |
| 辐射诱发肿瘤细胞旁效应的免疫学实验研究 |
| An immunological study on bystander effect of tumor cells induced by irradiation |
| 投稿时间：2003-10-27 |
| DOI： |
| 中文关键词: 自然杀伤细胞 免疫活性 辐射旁效应 肿瘤细胞Yac-Ⅰ |
| 英文关键词:Nature killer cells Immunological activation Bystander effect induced by irradiation Yac-Ⅰ tumor cells |
| 基金项目:国家自然科学基金资助项目(10105012) |
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| 中文摘要: |
| 目的 研究用自然杀伤(NK)细胞活性的变化检测肿瘤细胞YacⅠ在60Coγ射线照射后是否存在旁观者效应(旁效应),以及其旁效应剂量学和时间效应特征。方法 以健康ICR小鼠脾NK细胞活性为生物终点,检测NK细胞对不同条件培养肿瘤细胞YacⅠ的活性,用培养基介导法观察旁效应,同时,培养不同时间观察其时间规律。结果 每个剂量组NK细胞活性的均值与对照细胞NK细胞活性均值差异有显著性(P<005)。照射后分别培养0和24h,各剂量组间的NK细胞活性均值差异无显著性。结论 60Coγ射线照射能诱发肿瘤细胞YacⅠ的旁效应。旁效应在低剂量时较为明显。 |
| 英文摘要: |
| Objective To study the bystander effect induced by γ-irradiation through testing the activation of nature killer (NK) cells. Methods In this paper, the tested biological endpoint was the activation of NK cells against Yac-Ⅰ tumor cells, and traditional culture medium-mediate method was chosen to detect the bystander effect. Results The differences of the NK cell activation between cells of the control group and each dose group were all significant (P <0.05). The dose group that showed the strongest NK cell activation was 0.5 Gy. In the irradiation groups, no NK cell activation increase was found along with the increasing dose. Conclusion 60 Co γ-irradiation can trigger the bystander effect of Yac-Ⅰ tumor cell. Some of these data are compatible with those of the relative studies. |
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