| 陈俊,张积仁,肖明星,余志坚,陈春.P糖蛋白抑制X射线诱导凋亡及对线粒体膜电位的影响[J].中华放射医学与防护杂志,2004,24(4):297-299 |
| P糖蛋白抑制X射线诱导凋亡及对线粒体膜电位的影响 |
| P-glycoprotein down-regulates the X-ray-induced apoptosis of a drug-resistant tumor cell line MCF-7/Adr |
| 投稿时间：2004-01-18 |
| DOI： |
| 中文关键词: P糖蛋白 线粒体膜电位 细胞凋亡 X射线 |
| 英文关键词:P-gp Mitochondrial membrane potenial Apoptosis X-ray |
| 基金项目:国家自然科学基金资助项目(30100045) |
| 作者 | 单位 | | 陈俊 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | 张积仁 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | 肖明星 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | 余志坚 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | 陈春 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China |
|
| 摘要点击次数: 2357 |
| 全文下载次数: 1476 |
| 中文摘要: |
| 目的 观察P糖蛋白(Pgp)对X射线诱导凋亡和线粒体膜电位(ΔΨm)的影响。方法 运用流式细胞仪检测X射线照射后不同时间点细胞凋亡率和ΔΨm的动态变化。结果 Pgp功能抑制组细胞在X射线照射后6、12、24h细胞凋亡率分别为25.53%±2.85%,30.43%±2.21%和3903%±260%;对照组分别是16.13%±1.16%,21.73%±1.31%和27.53%±2.55%。抑制组显著高于对照组(P<001)。在X射线照射后6、12、24hPgp抑制组和对照组细胞ΔΨm平均荧光强度(MIF)均较照射前降低,但Pgp抑制组各时间点ΔΨmMIF值较对照组细胞下降更显著(P<001)。结论 P糖蛋白对X射线诱导凋亡具有显著抑制效应,其机理可能和其稳定的ΔΨm有关。 |
| 英文摘要: |
| Objective To investigate the regulatory effect of P-gp on X-ray-induced apoptosis of drug-resistant tumor cells. Methods Anti-P-gp McAb was applied to block P-gp function. MCF-7/Adr, a P-gp-overexpressing drug-resistant breast cancer cell line; was irradiated with X-rays. Flow cytometry was performed to examine dynamic changes of apoptotic ratio and mitochondrial membrane potenial(Δ Ψm)at various times after X-ray irradiation. Results Apoptotic ratio of the P-gp-blocked group was 25.53%±2.85%;30.43%±2.21%;39.03%±2.60%, and of the control group was 16.13%±1.16%;21.73%±1.31%;27.53%±2.55% at 6 h, 12 h, 24 h, respectively, after X-ray irradiation.The apoptotic ratio of the P-gp-blocked group was significantly up-regulated, as compared with that of the control group( P <0.01). The mitochondrial membrane potenial(Δ Ψm) of both the P-gp-blocked group and the control group was decreased, but the Δ Ψm of the P-gp-blocked group was significantly lower than that of the control group (P < 0.01). Conclusion P-gp down-regulates the X-ray-induced apoptosis of the drug-resistant MCF-7/Adr cells and elevates the mitochondrial membrane potenial(Δ Ψm). |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|