中华放射医学与防护杂志

鞠桂芝,付士波,闫凤琴.电离辐射诱导细胞周期解偶联的实验研究[J].中华放射医学与防护杂志,2004,24(4):295-296

电离辐射诱导细胞周期解偶联的实验研究

Experimental study on cell cycle uncoupling induced by ionizing radiation

投稿时间：2003-11-05

DOI：

英文关键词:Ionizing radiation Cell cycle uncoupling

基金项目:国家自然科学基金资助项目(30270346)

作者	单位
鞠桂芝	MH of Radiobiology Research Unit, Medical Center in Memorial of Norman Bethune of Jilin University, Changchun 130021, China
付士波	MH of Radiobiology Research Unit, Medical Center in Memorial of Norman Bethune of Jilin University, Changchun 130021, China
闫凤琴	MH of Radiobiology Research Unit, Medical Center in Memorial of Norman Bethune of Jilin University, Changchun 130021, China

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中文摘要:

目的观察电离辐射能否诱导细胞周期解偶联。方法采用PI荧光标记及流式细胞术(FCM)测定细胞周期并分析细胞倍体的变化。结果 10、20、40和6.0GyX射线照射后24h,SKOV3的二倍体细胞数明显减少(分别为P<0.01),且呈现明显的剂量依赖性;而四倍体细胞数明显增加(分别为P<0.01),亦呈现明显的剂量依赖性;与此同时,八倍体细胞数显著增高(分别为P<0.01)。结论电离辐射可诱导人卵巢癌SKOV3细胞周期解偶联。

英文摘要:

Objective To investigate whether or not cell cycle uncoupling could be induced by ionizing radiation. Methods Cell cycle was analyzed by flow cytometry (FCM) following staining of cells with propidium iodide (PI) and polyploid cells were analyzed. Results It was found that 24 h after exposure of SKOV-3 cells to X-rays at the doses of 1.0, 2.0, 4.0 or 6.0 Gy, the 2 N cells were decreased significantly (P <0.01 respectively), whereas both 4 N and 8 N cells were increased significantly ( P <0.01 for all cases). Conclusion Cell cycle uncoupling could be induced by ionizing radiation.

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