| 李体远,张英男,黄瑞芳,郑向鹏,郭一玲,顾望为,林继红.增敏(89)Sr内照射时小鼠骨髓DNA损伤的实验研究[J].中华放射医学与防护杂志,2004,24(3):247-248 |
| 增敏(89)Sr内照射时小鼠骨髓DNA损伤的实验研究 |
| Influence of 89Sr therapy with radiosensitizer on DNA damage of bone marrow in mice |
| 投稿时间：2003-12-26 |
| DOI： |
| 中文关键词: DNA损伤 89Sr 烟酰胺 Carbogen 骨髓 微核 |
| 英文关键词:DNA damage 89Sr Nicotinamide Carbogen Bone marrow Micronuclei |
| 基金项目:广东省自然科学基金资助项目(000372) |
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| 中文摘要: |
| 目的 探讨89Sr内照射治疗时增敏剂烟酰胺和Carbogen对小鼠骨髓网织红细胞DNA损伤的影响。方法 昆明系小鼠, 按组注射烟酰胺和(或)吸入气体Carbogen,对照组注射同体积生理盐水。经小鼠尾静脉“弹丸”注射200 μl89SrCl,放射性活度7400 kBq(200μCi).正常组注射同体积生理盐水。分别于注射后第1,2,3,4,6,8,15,20,30,60和90天批量处死小鼠, 立即分离股骨骨髓, 荧光法进行小鼠骨髓网织红细胞微核测定。结果 治疗组微核出现频率随观察时间呈双峰, 各组第1峰出现时间在第2～4天, 第2峰在第10～14天。阴性对照和阳性对照组比较, 微核产生频率差异有显着性(F=15517,P<0.001).其余各组(89Sr+N+C,89Sr,89Sr+N和89Sr+C)间相比较差异无显着性(F=0717,P>0.05).与89Sr组比较, 单微核、双微核及三微核形成率差异均无显着性。未观察到四微核细胞。结论 增敏剂烟酰胺和Carbogen的应用在增敏89Sr治疗时不增加89Sr对小鼠的损伤。 |
| 英文摘要: |
| Objective To study the influence of 89 Sr therapy with radiosensitizer on DNA damage of bone marrow in mice. Methods Chinese Kunming mice were divided into 5 groups respectively:negative control (saline),positive control ( 89 Sr), 89 Sr+nicotinamide, 89 Sr+Carbogen and 89 Sr+nicotinamide+carbogen groups. 89 SrCl was intravenously injected as a blous containing activites of 7400 kBq(200 μCi)200μl and saline was administered in equal volume in the negative control group.On days 1,2,3,4,6,8,15,20,30,60 and 90 after injection,mice were sacrificed for micronuclei assay in femoral marrow reticulocytes (MnRETs).One thousand randomly selected reticulocytes from each mouse were scored for the number of micronuclei. Results The average frequency of MnRETs induced as a function of time after injection is shown in a dual peak curve.The first maximum MnRETs frequency occurred between the 2nd and the 4th day,and the second between the 10th and the 14th day.A significant statistic difference on the MnRETs frequency was found between the negative and the positive control groups(P<0.001),while no differences were noted among 89 Sr+N+C, 89 Sr, 89 Sr+N and 89 Sr+C groups (P>0.05).There were also no significant differences on single,double or ternary micronuclei cell frequencies among 89 Sr+N+C, 89 Sr, 89 Sr+N and 89 Sr+C groups ( P>0.05).No tetrad microncuclei cell was found in the experiment. Conclusion Administration of radiosensitizer does not aggravate the toxicity on bone marrow. |
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