中华放射医学与防护杂志

刘龙,刘璐,童冠圣,等.⁽³²⁾P-磷酸铬体外诱导人非小细胞肺癌A549细胞凋亡[J].中华放射医学与防护杂志,2004,24(3):234-236.LIU Long,LIU Lu,TONG Guan-sheng,et al.Study on apoptosis of human non-small cell pulmonary carcinoma A549 cells induced by ³²P-chromium phosphates in vitro[J].Chin J Radiol Med Prot,2004,24(3):234-236

⁽³²⁾P-磷酸铬体外诱导人非小细胞肺癌A549细胞凋亡

Study on apoptosis of human non-small cell pulmonary carcinoma A549 cells induced by ³²P-chromium phosphates in vitro

投稿时间：2004-01-15

DOI：

中文关键词: 肿瘤非小细胞肺癌细胞凋亡放射性核素 ³²P-磷酸铬

英文关键词:Tumor Non-small cell lung cancer Apoptosis Radioactive nucleotied ³²P chromium phosphate

基金项目:江苏省科委应用基础基金资助项目(BJ2000004); 江苏省卫生厅科研基金资助项目(H200117)

作者	单位	E-mail
刘龙	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室	luliuzhou.@gina.com
刘璐	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室
童冠圣	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室
封国生	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室
高宏	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室
高文	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室
李澄	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室
刘志勇	210009 南京东南大学核医学技术研究所铁道部北京铁路总医院ICU室

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中文摘要:

目的观察³²P磷酸铬(³²P胶体)体外诱导人非小细胞肺癌(NSCLC)A549细胞周期变化和发生细胞凋亡的现象, 建立剂量效应和时间效应关系。方法将³²P胶体加入A549细胞培养体系进行内照射, 初始放射性浓度分别为0,93,180,278,370和463MBqL,采用Giemsa染色、透射电镜、末端原位标记(TUNEL)等方法进行凋亡细胞的形态学、超微病理、生化特征检测, 应用流式细胞术定量检测细胞周期变化和细胞凋亡率。结果 A549细胞受照射后S期+G²M期细胞比率在96h以内呈上升趋势, 之后逐渐下降。A549细胞受照射后72h左右出现兴奋性表现, 96h起各受照组均检出凋亡, 各剂量组在照射后120h达到凋亡高峰。结论在较低剂量范围内, ³²P胶体内照射可以体外诱导人NSCLCA549发生72h以后的迟发相凋亡, 细胞凋亡率与内照射初始放射性浓度呈正相关。

英文摘要:

Objective To observe the phasic change and apoptosis of A549 non-small cell lung cancer cells induced by ^{32^{P-chromium phosphate in vitro ,and establish the dose-response and time-response relationship. Methods Internal irradiation was conducted by adding 32 P colloid into A549 cell culture system.The initial radioactivities were 0,93,180,278,370,463 MBqL,respectively.Giemsa stain,transmission electron microscopy and TUNEL were used in assessing morphologic,ultra structural pathologic and biochemical characteristics,and the phasic changes and apoptotic rates of cells were studied by flow cytometry. Results After irradiation of A549 cells,the cell ratio of S+G₂-M phase tended to increase within 96 h,then decreased gradually.At 72 h after irradiation the A549 cells showed excited manifestation,and in each irradiation group apoptosis began from 96 h of irradiation,and attained the peak at 120 h. Conclusion In the lower dosagerange, ³² P internal irradiation may induce human NSCLC A549 cells to present delayed onset of apoptosis,and the rate of cell apoptosis is positively correlated to the initial radioactivity concentration.}}

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