| 蒲晓允,陈晓莉,潘静,李招权,邓均,黄辉,叶勇.6 Gy γ射线对骨髓细胞生物力学特性的影响[J].中华放射医学与防护杂志,2004,24(3):231-233 |
| 6 Gy γ射线对骨髓细胞生物力学特性的影响 |
| The affect of bone marrow cell biomechanical characteristics to 6 Gy γ irradiation-injured mice |
| 投稿时间：2003-01-18 |
| DOI： |
| 中文关键词: 生物力学特性 骨髓细胞 黏附性 表面电荷 变形性 |
| 英文关键词:Biomechanical characteristics Bone marrow cells Adhesiveness Surface charge Deformability |
| 基金项目:全军“九五”攻关课题基金资助项目 |
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| 中文摘要: |
| 目的 了解放射损伤条件下骨髓细胞(包括基质细胞和造血细胞)生物力学特性的改变。方法 以6Gy60Coγ射线照射昆明系小鼠制备动物模型, 以细胞电泳法检测骨髓细胞的细胞电泳率, 荧光探针法测定细胞膜微黏度, 旋转法检测细胞的黏附性, 核孔滤膜法检测细胞的变形指数。结果 放射损伤后骨髓细胞的变形能力降低, 细胞膜微黏度增加, 细胞的黏附率和电泳率显着降低。结论 6Gy60Coγ射线对骨髓细胞的生物力学特性有显着影响, 可能是放射损伤后造血功能减退的原因之一。 |
| 英文摘要: |
| Objective To explore the change of bone marrow cell biomechanical characteristics in radiation-injured mice and the influencing factors. Methods Male Kumming mice were exposed to total body irradiation of 6 Gy γ-rays from a 60Co source.Electrophoresis,DPH probe,micropore filter,and adhesion rate methods were used to detect cell surface charge,membrane microviscosity,cell deformability,and cell adhesion,respectively. Results The deformability,adhesiveness and cell surface charges of bone marrow cells(including hematopoietic cells and stromal cells)were dramatically decreased,but membrane microviscosity was obviously increased after irradiation on 1 d,3 d and 7 d. Conclusion The biomechanical characteristics of bone marrow cells are obviously changed after radiation injury.It might be one of the reasons of hematopoietic failure after irradiation. |
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