| 李邦印,应万涛,戴为民,徐勤枝,霍艳英,胡迎春,张开泰,吴德昌.MTAP在人BEP2D细胞辐射致癌模型和肺癌中表达研究[J].中华放射医学与防护杂志,2004,24(3):222-225 |
| MTAP在人BEP2D细胞辐射致癌模型和肺癌中表达研究 |
| Down-regulation of methylthioadenosine phosphorylase in malignant BERP35T-2 cells and in non-small cell lung cancers |
| 投稿时间：2003-08-28 |
| DOI： |
| 中文关键词: α粒子 甲硫腺苷磷酸化酶 非小细胞肺癌 |
| 英文关键词:α particles MTAP NSCLC |
| 基金项目:国家重点基础研究发展规划973基金资助项目(G1998051207) |
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| 中文摘要: |
| 目的 探索甲硫腺苷磷酸化酶(Methylthioadenosine Phosphorylase,MTAP)在人支气管上皮BEP2D永生化细胞的恶性转化过程和临床非小细胞肺癌中的表达变化。方法 培养BEP2D和BERP35T2细胞, 制备细胞总蛋白, 进行双相电泳和质谱分析;选择有表达差异的蛋白质点MTAP,在细胞模型中进行Northern Blot分析;对获得的15例肺癌标本用RT-PCR方法分析验证MTAP的表达水平。结果 双相电泳发现MTAP在恶性转化细胞BERP35T-2中表达降低;Northern Blot分析表明MTAP mRNA在BEP2D永生化细胞中表达水平很高, 而在BERP35T-2恶转细胞中表达极低;对15例非小细胞肺癌进行RTPCR分析, 发现MTAP在733%(1115)的肿瘤组织中低表达;在中低分化组肺癌的低表达频率(90.9%)显着高于高分化组(25%),在腺癌与鳞癌组则没有显着性差异。结论 MTAP低表达现象与肺癌恶性转化密切相关, 可能发挥抑癌基因的生物学功能。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the expression alteration of MTAP gene in tumorgenesis of bronchial epithelial cell line and Chinese non-small lung cancers. Methods Total protein from BEP2-D and BERP35T-2 cells was extracted and analyzed by 2-D electrophoesis and peptide mass fingerprinting spectrum.Northern blot was performed to confirm differences of expression of MTAP gene between BEP2D and BERP35T-2.RT-PCR was used to observe the mRNA expression of MTAP in 15 clinical cases with non-small cell lung cancer. Results MTAP was found to be expressed at lower level in malignant BERP35T-2 cells than in BEP2D analyzed by 2-dimensional electrophoresis,which was confirmed at transcriptional level by Northern blot.In 11 out of 15 NSCLC samples analyzed by RT-PCR,MTAP expression was down-regulated.MTAP was poorly expressed in 90.9% of mildpoorly differentiated NSCLC cases,while in 25% of well-differentiated cases. Conclusion The result of this study indicates that the down-regulations of MTAP at protein level or mRNA level in Chinese NSCLC might contribute to the carcinogenesis of lung and MTAP might be a candidate of tumor suppressor gene. |
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